Neointimal hyperplasia and vascular endothelial growth factor expression are increased in normoglycemic, insulin resistant, obese fatty rats

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Abstract

Objective: Insulin resistance is associated with a constellation of factors that enhance the artherosclerotic process. Vessel injury results in the formation of a markedly increased neointima in type 2 diabetes. Increased neointimal hyperplasia (NH) and vascular endothelial growth factor (VEGF) expression may lead to restenosis post angioplasty. We studied NH and VEGF expression in an obese, insulin resistant, but normoglycemic rat model, after carotid balloon injury. Methods and results: Diabetic rats (ZDF, n = 10), normoglycemic, insulin-resistant rats (ZDF-normoglycemic, n = 6) as well as Zucker fatty rats (FZ, n = 6), and lean Zucker rats (LZ, n = 6), all 13-16 weeks old, were subjected to right carotid injury by an angioplasty catheter introduced via the femoral artery. Three weeks later the rats were sacrificed and serum and carotids obtained. The intima-media ratio (I/M) was then calculated. ZDF-normoglycemic, FZ and ZDF-diabetic rats were all hyperinsulinemic and hypertriglyceridemic when compared to LZ rats. ZDF diabetic rats were hyperglycemic while FZ, ZDF-normoglycemic and LZ rats were normoglycemic. The I/M ratio for ZDF and FZ rats were significantly greater than for LZ rats. The VEGF expression was significantly greater in ZDF and FZ rats than LZ rats. Conclusions: In conclusion, insulin resistance increases neointimal hyperplasia and VEGF expression even with normoglycemia, after carotid angioplasty in rats.

Original languageEnglish (US)
Pages (from-to)283-289
Number of pages7
JournalAtherosclerosis
Volume184
Issue number2
DOIs
StatePublished - Feb 1 2006

Fingerprint

Vascular Endothelial Growth Factor A
Hyperplasia
Insulin
Angioplasty
Zucker Rats
Insulin Resistance
Wounds and Injuries
Neointima
Femoral Artery
Type 2 Diabetes Mellitus
Catheters

Keywords

  • Hyperinsulinemia
  • Insulin resistance
  • Neointimal hyperplasia
  • Type 2 diabetes
  • Vascular endothelial growth factor
  • Vascular injury

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

@article{c35c564daeb6483ea2c0ec2143564c1f,
title = "Neointimal hyperplasia and vascular endothelial growth factor expression are increased in normoglycemic, insulin resistant, obese fatty rats",
abstract = "Objective: Insulin resistance is associated with a constellation of factors that enhance the artherosclerotic process. Vessel injury results in the formation of a markedly increased neointima in type 2 diabetes. Increased neointimal hyperplasia (NH) and vascular endothelial growth factor (VEGF) expression may lead to restenosis post angioplasty. We studied NH and VEGF expression in an obese, insulin resistant, but normoglycemic rat model, after carotid balloon injury. Methods and results: Diabetic rats (ZDF, n = 10), normoglycemic, insulin-resistant rats (ZDF-normoglycemic, n = 6) as well as Zucker fatty rats (FZ, n = 6), and lean Zucker rats (LZ, n = 6), all 13-16 weeks old, were subjected to right carotid injury by an angioplasty catheter introduced via the femoral artery. Three weeks later the rats were sacrificed and serum and carotids obtained. The intima-media ratio (I/M) was then calculated. ZDF-normoglycemic, FZ and ZDF-diabetic rats were all hyperinsulinemic and hypertriglyceridemic when compared to LZ rats. ZDF diabetic rats were hyperglycemic while FZ, ZDF-normoglycemic and LZ rats were normoglycemic. The I/M ratio for ZDF and FZ rats were significantly greater than for LZ rats. The VEGF expression was significantly greater in ZDF and FZ rats than LZ rats. Conclusions: In conclusion, insulin resistance increases neointimal hyperplasia and VEGF expression even with normoglycemia, after carotid angioplasty in rats.",
keywords = "Hyperinsulinemia, Insulin resistance, Neointimal hyperplasia, Type 2 diabetes, Vascular endothelial growth factor, Vascular injury",
author = "Desouza, {Cyrus V} and Moira Gerety and Hamel, {Frederick G}",
year = "2006",
month = "2",
day = "1",
doi = "10.1016/j.atherosclerosis.2005.04.015",
language = "English (US)",
volume = "184",
pages = "283--289",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "2",

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TY - JOUR

T1 - Neointimal hyperplasia and vascular endothelial growth factor expression are increased in normoglycemic, insulin resistant, obese fatty rats

AU - Desouza, Cyrus V

AU - Gerety, Moira

AU - Hamel, Frederick G

PY - 2006/2/1

Y1 - 2006/2/1

N2 - Objective: Insulin resistance is associated with a constellation of factors that enhance the artherosclerotic process. Vessel injury results in the formation of a markedly increased neointima in type 2 diabetes. Increased neointimal hyperplasia (NH) and vascular endothelial growth factor (VEGF) expression may lead to restenosis post angioplasty. We studied NH and VEGF expression in an obese, insulin resistant, but normoglycemic rat model, after carotid balloon injury. Methods and results: Diabetic rats (ZDF, n = 10), normoglycemic, insulin-resistant rats (ZDF-normoglycemic, n = 6) as well as Zucker fatty rats (FZ, n = 6), and lean Zucker rats (LZ, n = 6), all 13-16 weeks old, were subjected to right carotid injury by an angioplasty catheter introduced via the femoral artery. Three weeks later the rats were sacrificed and serum and carotids obtained. The intima-media ratio (I/M) was then calculated. ZDF-normoglycemic, FZ and ZDF-diabetic rats were all hyperinsulinemic and hypertriglyceridemic when compared to LZ rats. ZDF diabetic rats were hyperglycemic while FZ, ZDF-normoglycemic and LZ rats were normoglycemic. The I/M ratio for ZDF and FZ rats were significantly greater than for LZ rats. The VEGF expression was significantly greater in ZDF and FZ rats than LZ rats. Conclusions: In conclusion, insulin resistance increases neointimal hyperplasia and VEGF expression even with normoglycemia, after carotid angioplasty in rats.

AB - Objective: Insulin resistance is associated with a constellation of factors that enhance the artherosclerotic process. Vessel injury results in the formation of a markedly increased neointima in type 2 diabetes. Increased neointimal hyperplasia (NH) and vascular endothelial growth factor (VEGF) expression may lead to restenosis post angioplasty. We studied NH and VEGF expression in an obese, insulin resistant, but normoglycemic rat model, after carotid balloon injury. Methods and results: Diabetic rats (ZDF, n = 10), normoglycemic, insulin-resistant rats (ZDF-normoglycemic, n = 6) as well as Zucker fatty rats (FZ, n = 6), and lean Zucker rats (LZ, n = 6), all 13-16 weeks old, were subjected to right carotid injury by an angioplasty catheter introduced via the femoral artery. Three weeks later the rats were sacrificed and serum and carotids obtained. The intima-media ratio (I/M) was then calculated. ZDF-normoglycemic, FZ and ZDF-diabetic rats were all hyperinsulinemic and hypertriglyceridemic when compared to LZ rats. ZDF diabetic rats were hyperglycemic while FZ, ZDF-normoglycemic and LZ rats were normoglycemic. The I/M ratio for ZDF and FZ rats were significantly greater than for LZ rats. The VEGF expression was significantly greater in ZDF and FZ rats than LZ rats. Conclusions: In conclusion, insulin resistance increases neointimal hyperplasia and VEGF expression even with normoglycemia, after carotid angioplasty in rats.

KW - Hyperinsulinemia

KW - Insulin resistance

KW - Neointimal hyperplasia

KW - Type 2 diabetes

KW - Vascular endothelial growth factor

KW - Vascular injury

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