Nematode Spatial and Ecological Patterns from Tropical and Temperate Rainforests

Dorota L. Porazinska, Robin M. Giblin-Davis, Thomas O. Powers, W. Kelley Thomas

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Large scale diversity patterns are well established for terrestrial macrobiota (e.g. plants and vertebrates), but not for microscopic organisms (e.g. nematodes). Due to small size, high abundance, and extensive dispersal, microbiota are assumed to exhibit cosmopolitan distributions with no biogeographical patterns. This assumption has been extrapolated from local spatial scale studies of a few taxonomic groups utilizing morphological approaches. Recent molecularly-based studies, however, suggest something quite opposite. Nematodes are the most abundant metazoans on earth, but their diversity patterns are largely unknown. We conducted a survey of nematode diversity within three vertical strata (soil, litter, and canopy) of rainforests at two contrasting latitudes in the North American meridian (temperate: the Olympic National Forest, WA, U.S.A and tropical: La Selva Biological Station, Costa Rica) using standardized sampling designs and sample processing protocols. To describe nematode diversity, we applied an ecometagenetic approach using 454 pyrosequencing. We observed that: 1) nematode communities were unique without even a single common species between the two rainforests, 2) nematode communities were unique among habitats in both rainforests, 3) total species richness was 300% more in the tropical than in the temperate rainforest, 4) 80% of the species in the temperate rainforest resided in the soil, whereas only 20% in the tropics, 5) more than 90% of identified species were novel. Overall, our data provided no support for cosmopolitanism at both local (habitats) and large (rainforests) spatial scales. In addition, our data indicated that biogeographical patterns typical of macrobiota also exist for microbiota.

Original languageEnglish (US)
Article numbere44641
JournalPloS one
Volume7
Issue number9
DOIs
StatePublished - Sep 11 2012

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rain forests
Nematoda
Soils
Tropics
Earth (planet)
Microbiota
Sampling
Olympic National Forest
Ecosystem
Soil
Processing
Costa Rica
Meridians
habitats
Vertebrates
Rainforest
soil
tropics
vertebrates
canopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Nematode Spatial and Ecological Patterns from Tropical and Temperate Rainforests. / Porazinska, Dorota L.; Giblin-Davis, Robin M.; Powers, Thomas O.; Thomas, W. Kelley.

In: PloS one, Vol. 7, No. 9, e44641, 11.09.2012.

Research output: Contribution to journalArticle

Porazinska, Dorota L. ; Giblin-Davis, Robin M. ; Powers, Thomas O. ; Thomas, W. Kelley. / Nematode Spatial and Ecological Patterns from Tropical and Temperate Rainforests. In: PloS one. 2012 ; Vol. 7, No. 9.
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