Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples

William J. Wolfgang, Teresa V. Passaretti, Reashma Jose, Jocelyn Cole, An Coorevits, Andrea N. Carpenter, Sherly Jose, Anita Van Landschoot, Jacques Izard, Donna J. Kohlerschmidt, Peter Vandamme, Floyd E. Dewhirst, Mark A. Fisher, Kimberlee A. Musser

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A polyphasic analysis was undertaken of seven independent isolates of Gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida and Illinois and healthy subgingival plaque from a patient in Virginia, USA. The 16S rRNA gene sequence similarity among these isolates was 99.7-100%, and the closest species with a validly published name was Neisseria lactamica (96.9% similarity to the type strain). DNA-DNA hybridization confirmed that these isolates are of the same species and are distinct from their nearest phylogenetic neighbour, N. lactamica. Phylogenetic analysis of 16S and 23S rRNA gene sequences indicated that the novel species belongs in the genus Neisseria. The predominant cellular fatty acids were C16:0, summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH) and C18:1 ω7c. The cellular fatty acid profile, together with other phenotypic characters, further supports the inclusion of the novel species in the genus Neisseria. The name Neisseria oralis sp. nov. (type strain 6332T =DSM 25276T =LMG 26725T) is proposed.

Original languageEnglish (US)
Pages (from-to)1323-1328
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART4
DOIs
StatePublished - Apr 1 2013

Fingerprint

Neisseria
Neisseria lactamica
rRNA Genes
Names
new species
Fatty Acids
ribosomal RNA
nucleotide sequences
fatty acid
Coccus
DNA
phylogeny
phylogenetics
nucleic acid hybridization
sampling
gene
fatty acid composition
fatty acids
analysis

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Wolfgang, W. J., Passaretti, T. V., Jose, R., Cole, J., Coorevits, A., Carpenter, A. N., ... Musser, K. A. (2013). Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples. International Journal of Systematic and Evolutionary Microbiology, 63(PART4), 1323-1328. https://doi.org/10.1099/ijs.0.041731-0

Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples. / Wolfgang, William J.; Passaretti, Teresa V.; Jose, Reashma; Cole, Jocelyn; Coorevits, An; Carpenter, Andrea N.; Jose, Sherly; Van Landschoot, Anita; Izard, Jacques; Kohlerschmidt, Donna J.; Vandamme, Peter; Dewhirst, Floyd E.; Fisher, Mark A.; Musser, Kimberlee A.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. PART4, 01.04.2013, p. 1323-1328.

Research output: Contribution to journalArticle

Wolfgang, WJ, Passaretti, TV, Jose, R, Cole, J, Coorevits, A, Carpenter, AN, Jose, S, Van Landschoot, A, Izard, J, Kohlerschmidt, DJ, Vandamme, P, Dewhirst, FE, Fisher, MA & Musser, KA 2013, 'Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples', International Journal of Systematic and Evolutionary Microbiology, vol. 63, no. PART4, pp. 1323-1328. https://doi.org/10.1099/ijs.0.041731-0
Wolfgang, William J. ; Passaretti, Teresa V. ; Jose, Reashma ; Cole, Jocelyn ; Coorevits, An ; Carpenter, Andrea N. ; Jose, Sherly ; Van Landschoot, Anita ; Izard, Jacques ; Kohlerschmidt, Donna J. ; Vandamme, Peter ; Dewhirst, Floyd E. ; Fisher, Mark A. ; Musser, Kimberlee A. / Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples. In: International Journal of Systematic and Evolutionary Microbiology. 2013 ; Vol. 63, No. PART4. pp. 1323-1328.
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