Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution

Michael D. Furtaw, Jon P. Anderson, Lyle R. Middendorf, Gregory R Bashford

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Fluorescence spectroscopy is used in many life science and clinical research diagnostic assays. Improvements in the sensitivity and limit-of-detection of these assays may have profound implications. Here, we demonstrate a near-infrared, surface-enhanced fluorescence technology that increases the signal of IRDye 800CW-labeled streptavidin by up to 2,530-fold while improving the limit-of-detection 1,000-fold. Citrate-stabilized, silver nanoparticles that aggregate in solution were used with the dye-protein conjugate to form plasmon-active nanostructures. The technique is straightforward to implement and fully compatible with commercially available immunoassay instrumentation and consumables.

Original languageEnglish (US)
Pages (from-to)27-34
Number of pages8
JournalPlasmonics
Volume9
Issue number1
DOIs
StatePublished - Feb 1 2014

Fingerprint

Silver
Nanoparticles
Limit of Detection
Assays
Fluorescence
Infrared radiation
Streptavidin
Biological Science Disciplines
Nanostructures
Fluorescence Spectrometry
Fluorescence spectroscopy
Immunoassay
Citric Acid
Coloring Agents
Technology
Research
Proteins
IRDye 800CW

Keywords

  • Aggregation
  • Near-infrared fluorescence
  • Plasmonics
  • Silver nanoparticles
  • Surface-enhanced fluorescence

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biochemistry

Cite this

Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution. / Furtaw, Michael D.; Anderson, Jon P.; Middendorf, Lyle R.; Bashford, Gregory R.

In: Plasmonics, Vol. 9, No. 1, 01.02.2014, p. 27-34.

Research output: Contribution to journalArticle

Furtaw, Michael D. ; Anderson, Jon P. ; Middendorf, Lyle R. ; Bashford, Gregory R. / Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution. In: Plasmonics. 2014 ; Vol. 9, No. 1. pp. 27-34.
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