Native cellular architecture of Treponema denticola revealed by cryo-electron tomography

Jacques Izard, Chyong Ere Hsieh, Ronald J. Limberger, Carmen A. Mannella, Michael Marko

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Using cryo-electron tomography, we are developing a refined description of native cellular structures in the pathogenic spirochete Treponema denticola. Tightly organized bundles of periplasmic flagella were readily observed in intact plunge-frozen cells. The periplasmic space was measured in both wild-type and aflagellate strains, and found to widen by less than the diameter of flagella when the latter are present. This suggests that a structural change occurs in the peptidoglycan layer to accommodate the presence of the flagella. In dividing cells, the flagellar filaments were found to bridge the cytoplasmic cylinder constriction site. Cytoplasmic filaments, adjacent to the inner membrane, run parallel to the tightly organized flagellar filaments. The cytoplasmic filaments may be anchored by a narrow plate-like structure. The tapering of the cell ends was conserved between cells, with a patella-shaped structure observed in the periplasm at the tip of each cytoplasmic cylinder. Several incompletely characterized structures have been observed in the periplasm between dividing cells, including a cable-like structure linking two cytoplasmic cylinders and complex foil-shaped structures.

Original languageEnglish (US)
Pages (from-to)10-17
Number of pages8
JournalJournal of Structural Biology
Volume163
Issue number1
DOIs
StatePublished - Jul 1 2008

Fingerprint

Treponema denticola
Electron Microscope Tomography
Periplasm
Flagella
Cytoskeleton
Spirochaetales
Patella
Peptidoglycan
Cellular Structures
Constriction
Membranes

Keywords

  • Cell division
  • Electron microscopy
  • Flagella
  • Intermediate-like filament
  • Motility
  • Septation
  • Spirochetes

ASJC Scopus subject areas

  • Structural Biology

Cite this

Native cellular architecture of Treponema denticola revealed by cryo-electron tomography. / Izard, Jacques; Hsieh, Chyong Ere; Limberger, Ronald J.; Mannella, Carmen A.; Marko, Michael.

In: Journal of Structural Biology, Vol. 163, No. 1, 01.07.2008, p. 10-17.

Research output: Contribution to journalArticle

Izard, Jacques ; Hsieh, Chyong Ere ; Limberger, Ronald J. ; Mannella, Carmen A. ; Marko, Michael. / Native cellular architecture of Treponema denticola revealed by cryo-electron tomography. In: Journal of Structural Biology. 2008 ; Vol. 163, No. 1. pp. 10-17.
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