Nanotopographic cell culture substrate

Polymer-demixed nanotextured films under cell culture conditions

Jung Y Lim, Christopher A. Siedlecki, Henry J. Donahue

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Modulating physical cell culture environments via nanoscale substrate topographic modification has recently been of significant interest in regenerative medicine. Many studies have utilized a polymer-demixing technique to produce nanotextured films and showed that cellular adhesion, proliferation, and differentiation could be regulated by the shape and scale of the polymer-demixed nanotopographies. However, little attention has been paid to the topographic fidelity of the polymer-demixed films when exposed to cell culture conditions. In this brief article, two polymer-demixing systems were employed to assess topographic changes in polymer-demixed films after fibronectin (FN) extracellular matrix protein adsorption and after incubation in phosphate-buffered saline at 37oC. We showed that FN adsorption induced very small variations (< 2 nm) to the polystyrene/polybromostyrene (PS/PBrS)-demixed nanoisland textures, not substantially altering the nanotopographies given by the polymer demixing. In addition, poly(L-lactic acid)/PS (PLLA/PS)-demixed nanoisland topographies using PLLA with Mw = 50×103 did not show notable degradation up to day 24.

Original languageEnglish (US)
Pages (from-to)252-255
Number of pages4
JournalBioResearch Open Access
Volume1
Issue number5
DOIs
StatePublished - Jan 1 2012

Fingerprint

Cell culture
Polymers
Cell Culture Techniques
Substrates
Fibronectins
Polymer films
Adsorption
Extracellular Matrix Proteins
Polystyrenes
Topography
Regenerative Medicine
Adhesion
Textures
Phosphates
Degradation
Cell Proliferation

Keywords

  • Cell culture
  • Degradation
  • Nanotopography
  • Polymer demixing
  • Protein adsorption

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nanotopographic cell culture substrate : Polymer-demixed nanotextured films under cell culture conditions. / Lim, Jung Y; Siedlecki, Christopher A.; Donahue, Henry J.

In: BioResearch Open Access, Vol. 1, No. 5, 01.01.2012, p. 252-255.

Research output: Contribution to journalArticle

Lim, Jung Y ; Siedlecki, Christopher A. ; Donahue, Henry J. / Nanotopographic cell culture substrate : Polymer-demixed nanotextured films under cell culture conditions. In: BioResearch Open Access. 2012 ; Vol. 1, No. 5. pp. 252-255.
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