Nanosecond pulsed electric field induced changes in cell surface charge density

Diganta Dutta, Xavier Lewis Palmer, Anthony Asmar, Michael Stacey, Shizhi Qian

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study reports that the surface charge density changes in Jurkat cells with the application of single 60 nanosecond pulse electric fields, using atomic force microscopy. Using an atomic force microscope tip and Jurkat cells on silica in a 0.01 M KCl ionic concentration, we were able to measure the interfacial forces, while also predicting surface charge densities of both Jurkat cell and silica surfaces. The most important finding is that the pulsing conditions varyingly reduced the cells’ surface charge density. This offers a novel way in which to examine cellular effects of pulsed electric fields that may lead to the identification of unique mechanical responses. Compared to a single low field strength NsPEF (15 kV/cm) application, exposure of Jurkat cells to a single high field strength NsPEF (60 kV/cm) resulted in a further reduction in charge density and major morphological changes. The structural, physical, and chemical properties of biological cells immensely influence their electrostatic force; we were able to investigate this through the use of atomic force microscopy by measuring the surface forces between the AFM's tip and the Jurkat cells under different pulsing conditions as well as the interfacial forces in ionic concentrations.

Original languageEnglish (US)
Pages (from-to)45-49
Number of pages5
JournalMicron
Volume100
DOIs
StatePublished - Sep 1 2017

Fingerprint

Jurkat Cells
Surface charge
Charge density
Electric fields
electric fields
cells
Surface Tension
Silicon Dioxide
Atomic Force Microscopy
Atomic force microscopy
Silica
atomic force microscopy
Electrostatic force
field strength
Chemical properties
silicon dioxide
Structural properties
Microscopes
Static Electricity
Physical properties

Keywords

  • Atomic force microscopy
  • Jurkat cell
  • Surface charge density

ASJC Scopus subject areas

  • Structural Biology
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Cell Biology

Cite this

Nanosecond pulsed electric field induced changes in cell surface charge density. / Dutta, Diganta; Palmer, Xavier Lewis; Asmar, Anthony; Stacey, Michael; Qian, Shizhi.

In: Micron, Vol. 100, 01.09.2017, p. 45-49.

Research output: Contribution to journalArticle

Dutta, Diganta ; Palmer, Xavier Lewis ; Asmar, Anthony ; Stacey, Michael ; Qian, Shizhi. / Nanosecond pulsed electric field induced changes in cell surface charge density. In: Micron. 2017 ; Vol. 100. pp. 45-49.
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