Nanoscale dynamics of centromere nucleosomes and the critical roles of CENP-A

Micah P. Stumme-Diers, Siddhartha Banerjee, Mohtadin Hashemi, Zhiqiang Sun, Yuri L Lyubchenko

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the absence of a functioning centromere, chromosome segregation becomes aberrant, leading to an increased rate of aneuploidy. The highly specific recognition of centromeres by kinetochores suggests that specific structural characteristics define this region, however, the structural details and mechanism underlying this recognition remains a matter of intense investigation. To address this, high-speed atomic force microscopy was used for direct visualization of the spontaneous dynamics of CENP-A nucleosomes at the sub-second time scale. We report that CENP-A nucleosomes change conformation spontaneously and reversibly, utilizing two major pathways: unwrapping, and looping of the DNA; enabling core transfer between neighboring DNA substrates. Along with these nucleosome dynamics we observed that CENP-A stabilizes the histone core against dissociating to histone subunits upon unwrapping DNA, unique from H3 cores which are only capable of such plasticity in the presence of remodeling factors. These findings have implications for the dynamics and integrity of nucleosomes at the centromere.

Original languageEnglish (US)
Pages (from-to)94-103
Number of pages10
JournalNucleic acids research
Volume46
Issue number1
DOIs
StatePublished - Jan 1 2018

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Nucleosomes
Centromere
Histones
DNA
Kinetochores
Chromosome Segregation
Atomic Force Microscopy
Aneuploidy
centromere protein A

ASJC Scopus subject areas

  • Genetics

Cite this

Nanoscale dynamics of centromere nucleosomes and the critical roles of CENP-A. / Stumme-Diers, Micah P.; Banerjee, Siddhartha; Hashemi, Mohtadin; Sun, Zhiqiang; Lyubchenko, Yuri L.

In: Nucleic acids research, Vol. 46, No. 1, 01.01.2018, p. 94-103.

Research output: Contribution to journalArticle

Stumme-Diers, Micah P. ; Banerjee, Siddhartha ; Hashemi, Mohtadin ; Sun, Zhiqiang ; Lyubchenko, Yuri L. / Nanoscale dynamics of centromere nucleosomes and the critical roles of CENP-A. In: Nucleic acids research. 2018 ; Vol. 46, No. 1. pp. 94-103.
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