Nanomechanical analysis of insulinoma cells after glucose and capsaicin stimulation using atomic force microscopy

Ruiguo Yang, Ning Xi, King Wai Chiu Lai, Bei Hua Zhong, Carmen Kar Man Fung, Chen Geng Qu, Donna H. Wang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aim: Glucose stimulates insulin secretion from pancreatic islet β cells by altering ion channel activity and membrane potential in the β cells. TRPV1 channel is expressed in the β cells and capsaicin induces insulin secretion similarly to glucose. This study aims to investigate the biophysical properties of the β cells upon stimulation of membrane channels using an atomic force microscopic (AFM) nanoindentation system.Methods:ATCC insulinoma cell line was used. Cell stiffness, a marker of reorganization of cell membrane and cytoskeleton due to ion channel activation, was measured in real time using an integrated AFM nanoindentation system. Cell height that represented structural changes was simultaneously recorded along with cell stiffness. Results: After administration of glucose (16, 20 and 40 mmol/L), the cell stiffness was markedly increased in a dose-dependent manner, whereas cell height was changed in an opposite way. Lower concentrations of capsaicin (1.67×10-9 and 1.67×10-8 mol/L) increased the cell stiffness without altering cell height. In contrast, higher concentrations of capsaicin (1.67×10-6 and 1.67×10-7 mol/L) had no effect on the cell physical properties.Conclusion:A unique bio-nanomechanical signature was identified for characterizing biophysical properties of insulinoma cells upon general or specific activation of membrane channels. This study may deepen our understanding of stimulus-secretion coupling of pancreatic islet cells that leads to insulin secretion.

Original languageEnglish (US)
Pages (from-to)853-860
Number of pages8
JournalActa Pharmacologica Sinica
Volume32
Issue number6
DOIs
StatePublished - Jun 1 2011
Externally publishedYes

Fingerprint

Insulinoma
Atomic Force Microscopy
Capsaicin
Glucose
Islets of Langerhans
Ion Channels
Insulin
Cytoskeleton
Membrane Potentials

Keywords

  • AFM nanoindentation
  • capsaicin
  • cellular height
  • cellular stiffness
  • glucose
  • insulin secretion

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Nanomechanical analysis of insulinoma cells after glucose and capsaicin stimulation using atomic force microscopy. / Yang, Ruiguo; Xi, Ning; Lai, King Wai Chiu; Zhong, Bei Hua; Fung, Carmen Kar Man; Qu, Chen Geng; Wang, Donna H.

In: Acta Pharmacologica Sinica, Vol. 32, No. 6, 01.06.2011, p. 853-860.

Research output: Contribution to journalArticle

Yang, Ruiguo ; Xi, Ning ; Lai, King Wai Chiu ; Zhong, Bei Hua ; Fung, Carmen Kar Man ; Qu, Chen Geng ; Wang, Donna H. / Nanomechanical analysis of insulinoma cells after glucose and capsaicin stimulation using atomic force microscopy. In: Acta Pharmacologica Sinica. 2011 ; Vol. 32, No. 6. pp. 853-860.
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abstract = "Aim: Glucose stimulates insulin secretion from pancreatic islet β cells by altering ion channel activity and membrane potential in the β cells. TRPV1 channel is expressed in the β cells and capsaicin induces insulin secretion similarly to glucose. This study aims to investigate the biophysical properties of the β cells upon stimulation of membrane channels using an atomic force microscopic (AFM) nanoindentation system.Methods:ATCC insulinoma cell line was used. Cell stiffness, a marker of reorganization of cell membrane and cytoskeleton due to ion channel activation, was measured in real time using an integrated AFM nanoindentation system. Cell height that represented structural changes was simultaneously recorded along with cell stiffness. Results: After administration of glucose (16, 20 and 40 mmol/L), the cell stiffness was markedly increased in a dose-dependent manner, whereas cell height was changed in an opposite way. Lower concentrations of capsaicin (1.67×10-9 and 1.67×10-8 mol/L) increased the cell stiffness without altering cell height. In contrast, higher concentrations of capsaicin (1.67×10-6 and 1.67×10-7 mol/L) had no effect on the cell physical properties.Conclusion:A unique bio-nanomechanical signature was identified for characterizing biophysical properties of insulinoma cells upon general or specific activation of membrane channels. This study may deepen our understanding of stimulus-secretion coupling of pancreatic islet cells that leads to insulin secretion.",
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N2 - Aim: Glucose stimulates insulin secretion from pancreatic islet β cells by altering ion channel activity and membrane potential in the β cells. TRPV1 channel is expressed in the β cells and capsaicin induces insulin secretion similarly to glucose. This study aims to investigate the biophysical properties of the β cells upon stimulation of membrane channels using an atomic force microscopic (AFM) nanoindentation system.Methods:ATCC insulinoma cell line was used. Cell stiffness, a marker of reorganization of cell membrane and cytoskeleton due to ion channel activation, was measured in real time using an integrated AFM nanoindentation system. Cell height that represented structural changes was simultaneously recorded along with cell stiffness. Results: After administration of glucose (16, 20 and 40 mmol/L), the cell stiffness was markedly increased in a dose-dependent manner, whereas cell height was changed in an opposite way. Lower concentrations of capsaicin (1.67×10-9 and 1.67×10-8 mol/L) increased the cell stiffness without altering cell height. In contrast, higher concentrations of capsaicin (1.67×10-6 and 1.67×10-7 mol/L) had no effect on the cell physical properties.Conclusion:A unique bio-nanomechanical signature was identified for characterizing biophysical properties of insulinoma cells upon general or specific activation of membrane channels. This study may deepen our understanding of stimulus-secretion coupling of pancreatic islet cells that leads to insulin secretion.

AB - Aim: Glucose stimulates insulin secretion from pancreatic islet β cells by altering ion channel activity and membrane potential in the β cells. TRPV1 channel is expressed in the β cells and capsaicin induces insulin secretion similarly to glucose. This study aims to investigate the biophysical properties of the β cells upon stimulation of membrane channels using an atomic force microscopic (AFM) nanoindentation system.Methods:ATCC insulinoma cell line was used. Cell stiffness, a marker of reorganization of cell membrane and cytoskeleton due to ion channel activation, was measured in real time using an integrated AFM nanoindentation system. Cell height that represented structural changes was simultaneously recorded along with cell stiffness. Results: After administration of glucose (16, 20 and 40 mmol/L), the cell stiffness was markedly increased in a dose-dependent manner, whereas cell height was changed in an opposite way. Lower concentrations of capsaicin (1.67×10-9 and 1.67×10-8 mol/L) increased the cell stiffness without altering cell height. In contrast, higher concentrations of capsaicin (1.67×10-6 and 1.67×10-7 mol/L) had no effect on the cell physical properties.Conclusion:A unique bio-nanomechanical signature was identified for characterizing biophysical properties of insulinoma cells upon general or specific activation of membrane channels. This study may deepen our understanding of stimulus-secretion coupling of pancreatic islet cells that leads to insulin secretion.

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