Nanoimaging for protein misfolding diseases

Research output: Contribution to journalReview article

32 Citations (Scopus)

Abstract

Misfolding and aggregation of proteins are widespread phenomena leading to the development of numerous neurodegenerative disorders such as Parkinson's, Alzheimer's, and Huntington's diseases. Each of these diseases is linked to structural misfolding and aggregation of a particular protein. The aggregated forms of the protein induce the development of a particular disease at all levels, leading to neuronal dysfunction and loss. Because protein refolding is frequently accompanied by transient association of partially folded intermediates, the propensity to aggregate is considered a general characteristic of the majority of proteins. X-ray crystallography, nuclear magnetic resonance, electron microscopy, and atomic force microscopy have provided important information on the structure of aggregates. However, fundamental questions, such as why the misfolded conformation of the protein is formed, and why this state is important for self-assembly, remain unanswered. Although it is well known that the same protein under pathological conditions can lead to the formation of aggregates with diverse biological consequences, the conditions leading to misfolding and the formation of the disease prone complexes are unclear, complicating any development of efficient prevention of the diseases. Misfolded states exist transiently, so answering these questions requires the use of novel approaches and methods. Progress has been made during the past few years, when recently developed ensemble methods and single-molecule biophysics techniques were applied to the problem of the protein misfolding. In this review, the impacts of these studies on the understanding of the mechanisms of the protein self-assembly into aggregates and on the development of treatments of the diseases are discussed.

Original languageEnglish (US)
Pages (from-to)526-543
Number of pages18
JournalWiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
Volume2
Issue number5
DOIs
StatePublished - Sep 1 2010

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Proteostasis Deficiencies
Proteins
Self assembly
Protein Refolding
Biophysics
Protein Conformation
Agglomeration
Atomic Force Microscopy
X Ray Crystallography
Huntington Disease
Neurodegenerative Diseases
Parkinson Disease
X ray crystallography
Electron Microscopy
Alzheimer Disease
Magnetic Resonance Spectroscopy
Electron microscopy
Conformations
Atomic force microscopy
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Nanoimaging for protein misfolding diseases. / Lyubchenko, Yuri L; Kim, Bo Hyun; Krasnoslobodtsev, Alexey V; Yu, Junping.

In: Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, Vol. 2, No. 5, 01.09.2010, p. 526-543.

Research output: Contribution to journalReview article

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