Nanofibrous Scaffolds with Biomimetic Composition for Skin Regeneration

Shahla Khalili, Saied Nouri Khorasani, Seyed Mohammad Razavi, Batool Hashemibeni, Ali Tamayol

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Treatments of skin injuries caused by trauma and diseases are among the most considerable medical problems. The use of scaffolds that can cover the wound area and support cellular ingrowth has shown great promise. However, mimicking the physicochemical properties of the native skin extracellular matrix (ECM) is essential for the successful integration of these scaffolds. Elastin has been known as the second main protein-based component of the native skin ECM. In this research, scaffolds containing gelatin, cellulose acetate, and elastin were fabricated using electrospinning. Subsequently, the effects of soluble elastin on the physical, mechanical, and biological properties of the prepared scaffolds were studied. The results confirmed that the presence of elastin in the composition changed the fiber morphology from straight to ribbon-like structure and decreased the swelling ratio and degradation rate of the scaffold. In vitro experiments showed that elastin-containing scaffolds supported the attachment and proliferation of fibroblast cells. Overall, the obtained results suggest the ternary blend of gelatin, cellulose acetate, and elastin as a good candidate for skin tissue engineering.

Original languageEnglish (US)
Pages (from-to)1193-1203
Number of pages11
JournalApplied Biochemistry and Biotechnology
Volume187
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Elastin
Biomimetics
Scaffolds (biology)
Regeneration
Skin
Scaffolds
Chemical analysis
Gelatin
Extracellular Matrix
Cellulose
Wounds and Injuries
Electrospinning
Fibroblasts
Tissue Engineering
Medical problems
Tissue engineering
Swelling
Cells
Cell Proliferation
Proteins

Keywords

  • Cellulose acetate
  • Elastin
  • Gelatin
  • Scaffolds
  • Skin tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Nanofibrous Scaffolds with Biomimetic Composition for Skin Regeneration. / Khalili, Shahla; Khorasani, Saied Nouri; Razavi, Seyed Mohammad; Hashemibeni, Batool; Tamayol, Ali.

In: Applied Biochemistry and Biotechnology, Vol. 187, No. 4, 01.04.2019, p. 1193-1203.

Research output: Contribution to journalArticle

Khalili, Shahla ; Khorasani, Saied Nouri ; Razavi, Seyed Mohammad ; Hashemibeni, Batool ; Tamayol, Ali. / Nanofibrous Scaffolds with Biomimetic Composition for Skin Regeneration. In: Applied Biochemistry and Biotechnology. 2019 ; Vol. 187, No. 4. pp. 1193-1203.
@article{c85978cefec3456fb017d3723b229128,
title = "Nanofibrous Scaffolds with Biomimetic Composition for Skin Regeneration",
abstract = "Treatments of skin injuries caused by trauma and diseases are among the most considerable medical problems. The use of scaffolds that can cover the wound area and support cellular ingrowth has shown great promise. However, mimicking the physicochemical properties of the native skin extracellular matrix (ECM) is essential for the successful integration of these scaffolds. Elastin has been known as the second main protein-based component of the native skin ECM. In this research, scaffolds containing gelatin, cellulose acetate, and elastin were fabricated using electrospinning. Subsequently, the effects of soluble elastin on the physical, mechanical, and biological properties of the prepared scaffolds were studied. The results confirmed that the presence of elastin in the composition changed the fiber morphology from straight to ribbon-like structure and decreased the swelling ratio and degradation rate of the scaffold. In vitro experiments showed that elastin-containing scaffolds supported the attachment and proliferation of fibroblast cells. Overall, the obtained results suggest the ternary blend of gelatin, cellulose acetate, and elastin as a good candidate for skin tissue engineering.",
keywords = "Cellulose acetate, Elastin, Gelatin, Scaffolds, Skin tissue engineering",
author = "Shahla Khalili and Khorasani, {Saied Nouri} and Razavi, {Seyed Mohammad} and Batool Hashemibeni and Ali Tamayol",
year = "2019",
month = "4",
day = "1",
doi = "10.1007/s12010-018-2871-7",
language = "English (US)",
volume = "187",
pages = "1193--1203",
journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",
issn = "0273-2289",
publisher = "Humana Press",
number = "4",

}

TY - JOUR

T1 - Nanofibrous Scaffolds with Biomimetic Composition for Skin Regeneration

AU - Khalili, Shahla

AU - Khorasani, Saied Nouri

AU - Razavi, Seyed Mohammad

AU - Hashemibeni, Batool

AU - Tamayol, Ali

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Treatments of skin injuries caused by trauma and diseases are among the most considerable medical problems. The use of scaffolds that can cover the wound area and support cellular ingrowth has shown great promise. However, mimicking the physicochemical properties of the native skin extracellular matrix (ECM) is essential for the successful integration of these scaffolds. Elastin has been known as the second main protein-based component of the native skin ECM. In this research, scaffolds containing gelatin, cellulose acetate, and elastin were fabricated using electrospinning. Subsequently, the effects of soluble elastin on the physical, mechanical, and biological properties of the prepared scaffolds were studied. The results confirmed that the presence of elastin in the composition changed the fiber morphology from straight to ribbon-like structure and decreased the swelling ratio and degradation rate of the scaffold. In vitro experiments showed that elastin-containing scaffolds supported the attachment and proliferation of fibroblast cells. Overall, the obtained results suggest the ternary blend of gelatin, cellulose acetate, and elastin as a good candidate for skin tissue engineering.

AB - Treatments of skin injuries caused by trauma and diseases are among the most considerable medical problems. The use of scaffolds that can cover the wound area and support cellular ingrowth has shown great promise. However, mimicking the physicochemical properties of the native skin extracellular matrix (ECM) is essential for the successful integration of these scaffolds. Elastin has been known as the second main protein-based component of the native skin ECM. In this research, scaffolds containing gelatin, cellulose acetate, and elastin were fabricated using electrospinning. Subsequently, the effects of soluble elastin on the physical, mechanical, and biological properties of the prepared scaffolds were studied. The results confirmed that the presence of elastin in the composition changed the fiber morphology from straight to ribbon-like structure and decreased the swelling ratio and degradation rate of the scaffold. In vitro experiments showed that elastin-containing scaffolds supported the attachment and proliferation of fibroblast cells. Overall, the obtained results suggest the ternary blend of gelatin, cellulose acetate, and elastin as a good candidate for skin tissue engineering.

KW - Cellulose acetate

KW - Elastin

KW - Gelatin

KW - Scaffolds

KW - Skin tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85053388382&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053388382&partnerID=8YFLogxK

U2 - 10.1007/s12010-018-2871-7

DO - 10.1007/s12010-018-2871-7

M3 - Article

VL - 187

SP - 1193

EP - 1203

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

SN - 0273-2289

IS - 4

ER -