Nanofiber scaffolds with gradients in mineral content for spatial control of osteogenesis

Wenying Liu, Justin Lipner, Jingwei Xie, Cionne N. Manning, Stavros Thomopoulos, Younan Xia

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Reattachment of tendon to bone has been a challenge in orthopedic surgery. The disparate mechanical properties of the two tissues make it difficult to achieve direct surgical repair of the tendon-to-bone insertion. Healing after surgical repair typically does not regenerate the natural attachment, a complex tissue that connects tendon and bone across a gradient in both mineral content and cell phenotypes. To facilitate the regeneration of the attachment, our groups have developed a nanofiber-based scaffold with a graded mineral coating to mimic the mineral composition of the native tendon-to-bone insertion. In the present work, we evaluated the ability of this scaffold to induce graded osteogenesis of adipose-derived mesenchymal stem cells (ASCs). Results from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and proliferating cell nuclear antigen staining indicated that cell proliferation was negatively correlated with the mineral content. In contrast, alkaline phosphatase staining, an indicator of osteogenesis, was positively correlated with the mineral content. Likewise, runt-related transcription factor 2 (an early marker of osteoblast differentiation) and osteocalcin (a late marker of osteoblast differentiation) immunostaining were both positively correlated with the mineral content. These results indicate that a gradient in mineral content on the surface of a nanofiber scaffold is capable of inducing graded differentiation of ASCs into osteoblasts for enthesis repair.

Original languageEnglish (US)
Pages (from-to)2842-2849
Number of pages8
JournalACS Applied Materials and Interfaces
Volume6
Issue number4
DOIs
StatePublished - Feb 26 2014

Fingerprint

Nanofibers
Osteogenesis
Scaffolds
Minerals
Tendons
Osteoblasts
Bone
Bone and Bones
Repair
Differentiation Antigens
Stem cells
Mesenchymal Stromal Cells
Tissue
Staining and Labeling
Transcription factors
Osteocalcin
Phosphatases
Proliferating Cell Nuclear Antigen
Orthopedics
Cell proliferation

Keywords

  • adipose-derived mesenchymal stem cell (ASC)
  • electrospun nanofiber
  • mineral gradient
  • osteogenesis
  • tendon-to-bone insertion
  • tissue engineering

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Nanofiber scaffolds with gradients in mineral content for spatial control of osteogenesis. / Liu, Wenying; Lipner, Justin; Xie, Jingwei; Manning, Cionne N.; Thomopoulos, Stavros; Xia, Younan.

In: ACS Applied Materials and Interfaces, Vol. 6, No. 4, 26.02.2014, p. 2842-2849.

Research output: Contribution to journalArticle

Liu, Wenying ; Lipner, Justin ; Xie, Jingwei ; Manning, Cionne N. ; Thomopoulos, Stavros ; Xia, Younan. / Nanofiber scaffolds with gradients in mineral content for spatial control of osteogenesis. In: ACS Applied Materials and Interfaces. 2014 ; Vol. 6, No. 4. pp. 2842-2849.
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