Nanofiber-based sutures induce endogenous antimicrobial peptide

Shixuan Chen, Liangpeng Ge, Adrian F. Gombart, Franklin D. Shuler, Mark Alan Carlson, Debra A Reilly, Jingwei Xie

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Aim: The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production. Methods: We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D3 (25D3). Results: The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D3 exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D3 and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D3 delivery alone. In addition, the 25D3/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60. Conclusion: The use of 25D3/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.

Original languageEnglish (US)
Pages (from-to)2597-2609
Number of pages13
JournalNanomedicine
Volume12
Issue number21
DOIs
Publication statusPublished - Nov 2017

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Keywords

  • 25-hydroxyvitamin D
  • co-delivery
  • electrospun nanofibers
  • pam3Cys-Ser-(Lys)4 peptide
  • surgical site infection
  • sutures

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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