Nanocarrier stimuli-activated gene delivery

Kenneth A. Howard, Mingdong Dong, David Oupicky, Harender S. Bisht, Claus Buss, Flemming Besenbacher, Jørgen Kjems

Research output: Contribution to journalReview article

39 Citations (Scopus)

Abstract

The nanoscale formation and activation of stimuli-responsive systems poly(N-isoproplyacrylamide)(PNIPA)- polyethylene(PEI)/DNA systems, was investigated using atomic force microscopy technique. The extracellular and intracellular behavior of nanocarriers was analyzed in response to thermal and redox stimuli. The thermoresponsive copolymers were prepared using PNIPA and single terminal carboxylic acid group conjugated with PEI. The relative content of NLS and HRP peptides in the synthesized copolypeptides was determined by amino acid analysis. Plasmid DNA/PEI-NIPA polyplexes nanocarriers were prepared with sodium acetate buffer (pH 5) by adding peptide solution into 150 mL of DNA in sodium acetate buffer. Nanocarriers were imagined using AFM under atmospheric conditions. It was observed that thermoresponsive nanocarriers showed increase in their size for site-specific deposition. Result shows that nanocarriers dissemble into the constituent components in intracellular condition.

Original languageEnglish (US)
Pages (from-to)54-57
Number of pages4
JournalSmall
Volume3
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Polyetherimides
Sodium Acetate
DNA
Genes
Peptides
Buffers
Sodium
Nuclear Localization Signals
Atomic Force Microscopy
Polyethylene
Carboxylic Acids
Carboxylic acids
Oxidation-Reduction
Polyethylenes
Amino acids
Atomic force microscopy
Plasmids
Copolymers
Hot Temperature
Chemical activation

Keywords

  • Atomic force microscopy
  • DNA
  • Gene delivery
  • Nanocarriers
  • Polymers

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Cite this

Howard, K. A., Dong, M., Oupicky, D., Bisht, H. S., Buss, C., Besenbacher, F., & Kjems, J. (2007). Nanocarrier stimuli-activated gene delivery. Small, 3(1), 54-57. https://doi.org/10.1002/smll.200600328

Nanocarrier stimuli-activated gene delivery. / Howard, Kenneth A.; Dong, Mingdong; Oupicky, David; Bisht, Harender S.; Buss, Claus; Besenbacher, Flemming; Kjems, Jørgen.

In: Small, Vol. 3, No. 1, 01.01.2007, p. 54-57.

Research output: Contribution to journalReview article

Howard, KA, Dong, M, Oupicky, D, Bisht, HS, Buss, C, Besenbacher, F & Kjems, J 2007, 'Nanocarrier stimuli-activated gene delivery', Small, vol. 3, no. 1, pp. 54-57. https://doi.org/10.1002/smll.200600328
Howard KA, Dong M, Oupicky D, Bisht HS, Buss C, Besenbacher F et al. Nanocarrier stimuli-activated gene delivery. Small. 2007 Jan 1;3(1):54-57. https://doi.org/10.1002/smll.200600328
Howard, Kenneth A. ; Dong, Mingdong ; Oupicky, David ; Bisht, Harender S. ; Buss, Claus ; Besenbacher, Flemming ; Kjems, Jørgen. / Nanocarrier stimuli-activated gene delivery. In: Small. 2007 ; Vol. 3, No. 1. pp. 54-57.
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