Nano-immunoassay with improved performance for detection of cancer biomarkers

Alexey V Krasnoslobodtsev, María P. Torres, Sukwinder Kaur, Ivan V. Vlassiouk, Robert J. Lipert, Maneesh Jain, Surinder Kumar Batra, Yuri L Lyubchenko

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Nano-immunoassay utilizing surface-enhanced Raman scattering (SERS) effect is a promising analytical technique for early detection of cancer. In its current standing the assay is capable of discriminating samples of healthy individuals from samples of pancreatic cancer patients. Further improvements in sensitivity and reproducibility will extend practical applications of the SERS-based detection platforms to wider range of problems. In this report, we discuss several strategies designed to improve performance of the SERS-based detection system. We demonstrate that reproducibility of the platform is enhanced by using atomically smooth mica surface as a template for preparation of capture surface in SERS sandwich immunoassay. Furthermore, assay's stability and sensitivity can be further improved by using either polymer or graphene monolayer as a thin protective layer applied on top of the assay addresses. The protective layer renders signal to be more stable against photo-induced damage and carbonaceous contamination. From the Clinical Editor: This study discusses strategies to improve the performance of nano-immunoassays utilizing surface-enhanced Raman scattering (SERS) effect , an analytical technique with high sensitivity that holds a great promise for early cancer biomarker detection.

Original languageEnglish (US)
Pages (from-to)167-173
Number of pages7
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Raman Spectrum Analysis
Tumor Biomarkers
Immunoassay
Raman scattering
Early Detection of Cancer
Assays
Graphite
Pancreatic Neoplasms
Polymers
Mica
Graphene
Monolayers
Contamination

Keywords

  • AFM
  • Cancer biomarkers
  • Nano-immunoassay
  • Nanodiagnostics
  • Pancreatic cancer
  • Surface-enhanced Raman scattering (SERS)

ASJC Scopus subject areas

  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Medicine (miscellaneous)
  • Pharmaceutical Science
  • Medicine(all)

Cite this

Nano-immunoassay with improved performance for detection of cancer biomarkers. / Krasnoslobodtsev, Alexey V; Torres, María P.; Kaur, Sukwinder; Vlassiouk, Ivan V.; Lipert, Robert J.; Jain, Maneesh; Batra, Surinder Kumar; Lyubchenko, Yuri L.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 11, No. 1, 01.01.2015, p. 167-173.

Research output: Contribution to journalArticle

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