N-linked oligosaccharides and metastatic propensity in in vivo selected mouse mammary adenocarcinoma cells

P. James Seberger, Eric M. Scholar, Linda Kelsey, William G Chaney, James E Talmadge

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Studies using metastatic variant selected in vivo from a cloned parental cell line demonstrate that the expression of β1-6 branched, N-linked carbohydrates and sialic acid were positively associated with in vitro invasiveness and inversely associated with metastatic potential, adherence, and in vivo growth rate. These results suggest that at least within one tumor model, a negative association occurs between metastatic potential and β1-6 branched oligosaccharide expression. In these studies two metastatic variants, Cl-66M1 and Cl-66M2, were selected following serial in vivo passage of Cl-66, a clonal cell line obtained from a mouse mammary adenocarcinoma cell line. The parent cell line and the two metastatic variants were approximately equal in their adherence to fibronectin, laminin, and collagen type IV coated plastic. In contrast, both Cl-66M1 and Cl-66M2 had a significantly increased ability to invade through matrigel invasion chambers and expressed significantly increased levels of β1-6 branched, N-linked carbohydrates, and sialic acid compared to the clonal parental cell line, Cl- 66. Furthermore, the in vivo tumor growth rates of these selected variants were decreased compared to Cl-66 with the longest tumor volume doubling time observed with Cl-66M2.

Original languageEnglish (US)
Pages (from-to)437-444
Number of pages8
JournalClinical and Experimental Metastasis
Volume17
Issue number5
DOIs
StatePublished - Dec 1 1999

Fingerprint

Oligosaccharides
Adenocarcinoma
Breast
Cell Line
N-Acetylneuraminic Acid
Carbohydrates
Collagen Type IV
Laminin
Growth
Tumor Burden
Fibronectins
Plastics
Neoplasms

Keywords

  • Breast cancer
  • Carbohydrate
  • Glycosylation
  • Invasion
  • Metastasis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

N-linked oligosaccharides and metastatic propensity in in vivo selected mouse mammary adenocarcinoma cells. / James Seberger, P.; Scholar, Eric M.; Kelsey, Linda; Chaney, William G; Talmadge, James E.

In: Clinical and Experimental Metastasis, Vol. 17, No. 5, 01.12.1999, p. 437-444.

Research output: Contribution to journalArticle

James Seberger, P. ; Scholar, Eric M. ; Kelsey, Linda ; Chaney, William G ; Talmadge, James E. / N-linked oligosaccharides and metastatic propensity in in vivo selected mouse mammary adenocarcinoma cells. In: Clinical and Experimental Metastasis. 1999 ; Vol. 17, No. 5. pp. 437-444.
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