N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent

Cristina De Castro, Immacolata Speciale, Garry Duncan, David D Dunigan, Irina V Agarkova, Rosa Lanzetta, Luisa Sturiale, Angelo Palmigiano, Domenico Garozzo, Antonio Molinaro, Michela Tonetti, James L Van Etten

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

N-glycosylation is a fundamental modification of proteins and exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N-glycan is herein described. This N-glycan core structure, common to all chloroviruses, is a pentasaccharide with a β-glucose linked to an asparagine residue which is not located in the typical sequon N-X-T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, which is in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semiconserved element because its absolute configuration is virus-dependent. Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus.

Original languageEnglish (US)
Pages (from-to)654-658
Number of pages5
JournalAngewandte Chemie - International Edition
Volume55
Issue number2
DOIs
StatePublished - Jan 11 2016

Fingerprint

Xylose
Viruses
Polysaccharides
Glucose
Fucose
Glycosylation
Rhamnose
Asparagine
Proteins
Galactose

Keywords

  • glycoproteins
  • glycosylations
  • natural products
  • structural biology
  • viruses

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent. / De Castro, Cristina; Speciale, Immacolata; Duncan, Garry; Dunigan, David D; Agarkova, Irina V; Lanzetta, Rosa; Sturiale, Luisa; Palmigiano, Angelo; Garozzo, Domenico; Molinaro, Antonio; Tonetti, Michela; Van Etten, James L.

In: Angewandte Chemie - International Edition, Vol. 55, No. 2, 11.01.2016, p. 654-658.

Research output: Contribution to journalArticle

De Castro, C, Speciale, I, Duncan, G, Dunigan, DD, Agarkova, IV, Lanzetta, R, Sturiale, L, Palmigiano, A, Garozzo, D, Molinaro, A, Tonetti, M & Van Etten, JL 2016, 'N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent', Angewandte Chemie - International Edition, vol. 55, no. 2, pp. 654-658. https://doi.org/10.1002/anie.201509150
De Castro, Cristina ; Speciale, Immacolata ; Duncan, Garry ; Dunigan, David D ; Agarkova, Irina V ; Lanzetta, Rosa ; Sturiale, Luisa ; Palmigiano, Angelo ; Garozzo, Domenico ; Molinaro, Antonio ; Tonetti, Michela ; Van Etten, James L. / N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent. In: Angewandte Chemie - International Edition. 2016 ; Vol. 55, No. 2. pp. 654-658.
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