N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent

Cristina De Castro, Immacolata Speciale, Garry Duncan, David D. Dunigan, Irina Agarkova, Rosa Lanzetta, Luisa Sturiale, Angelo Palmigiano, Domenico Garozzo, Antonio Molinaro, Michela Tonetti, James L. Van Etten

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

N-glycosylation is a fundamental modification of proteins and exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N-glycan is herein described. This N-glycan core structure, common to all chloroviruses, is a pentasaccharide with a β-glucose linked to an asparagine residue which is not located in the typical sequon N-X-T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, which is in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semiconserved element because its absolute configuration is virus-dependent. Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus.

Original languageEnglish (US)
Pages (from-to)654-658
Number of pages5
JournalAngewandte Chemie - International Edition
Volume55
Issue number2
DOIs
StatePublished - Jan 11 2016

Fingerprint

Xylose
Viruses
Polysaccharides
Glucose
Fucose
Glycosylation
Rhamnose
Asparagine
Proteins
Galactose

Keywords

  • glycoproteins
  • glycosylations
  • natural products
  • structural biology
  • viruses

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent. / De Castro, Cristina; Speciale, Immacolata; Duncan, Garry; Dunigan, David D.; Agarkova, Irina; Lanzetta, Rosa; Sturiale, Luisa; Palmigiano, Angelo; Garozzo, Domenico; Molinaro, Antonio; Tonetti, Michela; Van Etten, James L.

In: Angewandte Chemie - International Edition, Vol. 55, No. 2, 11.01.2016, p. 654-658.

Research output: Contribution to journalArticle

De Castro, C, Speciale, I, Duncan, G, Dunigan, DD, Agarkova, I, Lanzetta, R, Sturiale, L, Palmigiano, A, Garozzo, D, Molinaro, A, Tonetti, M & Van Etten, JL 2016, 'N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent', Angewandte Chemie - International Edition, vol. 55, no. 2, pp. 654-658. https://doi.org/10.1002/anie.201509150
De Castro, Cristina ; Speciale, Immacolata ; Duncan, Garry ; Dunigan, David D. ; Agarkova, Irina ; Lanzetta, Rosa ; Sturiale, Luisa ; Palmigiano, Angelo ; Garozzo, Domenico ; Molinaro, Antonio ; Tonetti, Michela ; Van Etten, James L. / N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent. In: Angewandte Chemie - International Edition. 2016 ; Vol. 55, No. 2. pp. 654-658.
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