N-Glycoproteomic Analysis of Chicken Egg Yolk

Fang Geng, Yunxiao Xie, Jinqiu Wang, Kaustav Majumder, Ning Qiu, Meihu Ma

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Posttranslational N-glycosylation of food proteins plays a critical role in their structure and function. However, the N-glycoproteome of chicken egg yolk (CEY) has not been studied yet. Glycopeptides hydrolyzed from CEY proteins were enriched, with deglycosylation occurring using PNGase F, and then were identified using a shotgun glycoproteomics strategy. A total of 217 N-glycosylation sites and 86 glycoproteins were identified in CEY, and these glycoproteins are mainly involved in the binding, biological regulation, catalytic activity, and metabolic processes. Among the identified CEY glycoproteins, 22 were recognized as proteases and protease inhibitors, suggesting that a proteinase/inhibitor regulation system exists in CEY; further, 15 were members of the complement and immune systems, which provide protection against potential threats during hatching. The study provides important structural information about CEY glycoproteins and aids in the understanding of the underlying mechanism of embryo development as well as changes in CEY functional characteristics during storage and processing.

Original languageEnglish (US)
JournalJournal of Agricultural and Food Chemistry
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Egg Yolk
chicken eggs
egg yolk
Chickens
Glycoproteins
Glycosylation
glycoproteins
Peptide Hydrolases
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Egg Proteins
glycosylation
Glycopeptides
proteinase inhibitors
Immune system
Protease Inhibitors
Catalyst activity
glycopeptides
yolk proteins
Firearms
catalytic activity

Keywords

  • N-glycosylation site
  • chicken egg yolk
  • glycoproteome
  • immunology
  • mass spectrometry
  • protease

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

N-Glycoproteomic Analysis of Chicken Egg Yolk. / Geng, Fang; Xie, Yunxiao; Wang, Jinqiu; Majumder, Kaustav; Qiu, Ning; Ma, Meihu.

In: Journal of Agricultural and Food Chemistry, 01.01.2018.

Research output: Contribution to journalArticle

Geng, Fang ; Xie, Yunxiao ; Wang, Jinqiu ; Majumder, Kaustav ; Qiu, Ning ; Ma, Meihu. / N-Glycoproteomic Analysis of Chicken Egg Yolk. In: Journal of Agricultural and Food Chemistry. 2018.
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