N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression

Marvin T. Nieman, Ryan S. Prudoff, Keith R. Johnson, Margaret J. Wheelock

Research output: Contribution to journalArticle

571 Citations (Scopus)

Abstract

E-cadherin is a transmembrane glycoprotein that mediates calcium- dependent, homotypic cell-cell adhesion and plays a role in maintaining the normal phenotype of epithelial cells. Decreased expression of E-cadherin has been correlated with increased invasiveness of breast cancer. In other systems, inappropriate expression of a nonepithelial cadherin, such as N- cadherin, by an epithelial cell has been shown to downregulate E-cadherin expression and to contribute to a scattered phenotype. In this study, we explored the possibility that expression of nonepithelial cadherins may be correlated with increased motility and invasion in breast cancer cells. We show that N-cadherin promotes motility and invasion; that decreased expression of E-cadherin does not necessarily correlate with motility or invasion; that N-cadherin expression correlates both with invasion and motility, and likely plays a direct role in promoting motility; that forced expression of E-cadherin in invasive, N-cadherin-positive cells does not reduce their motility or invasive capacity; that forced expression of N- cadherin in noninvasive, E-cadherin-positive cells produces an invasive cell' even though these cells continue to express high levels of E-cadherin; that N-cadherin-dependent motility may be mediated by FGF receptor signaling; and that cadherin-11 promotes epithelial cell motility in a manner similar to N- cadherin.

Original languageEnglish (US)
Pages (from-to)631-643
Number of pages13
JournalJournal of Cell Biology
Volume147
Issue number3
DOIs
StatePublished - Nov 1 1999

Fingerprint

Cadherins
Breast Neoplasms
Epithelial Cells
Phenotype
Fibroblast Growth Factor Receptors
Cell Adhesion
Cell Movement

Keywords

  • Breast cancer
  • E-cadherin
  • Fibroblast growth factor receptor
  • Motility
  • N-cadherin

ASJC Scopus subject areas

  • Cell Biology

Cite this

N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression. / Nieman, Marvin T.; Prudoff, Ryan S.; Johnson, Keith R.; Wheelock, Margaret J.

In: Journal of Cell Biology, Vol. 147, No. 3, 01.11.1999, p. 631-643.

Research output: Contribution to journalArticle

Nieman, Marvin T. ; Prudoff, Ryan S. ; Johnson, Keith R. ; Wheelock, Margaret J. / N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression. In: Journal of Cell Biology. 1999 ; Vol. 147, No. 3. pp. 631-643.
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