N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation

Sabine Puch, Sorin Armeanu, Christine Kibler, Keith R. Johnson, Claudia A. Müller, Margaret J. Wheelock, Gerd Klein

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The cadherins, an important family of cell adhesion molecules, are known to play major roles during embryonic development and in the maintenance of solid tissue architecture. In the hematopoietic system, however, little is known of the role of this cell adhesion family. By RT-PCR, western blot analysis and immunofluorescence staining we show that N-cadherin, a classical type I cadherin mainly expressed on neuronal, endothelial and muscle cells, is expressed on the cell surface of resident bone marrow stromal cells. FACS analysis of bone marrow mononuclear cells revealed that N-cadherin is also expressed on a subpopulation of early hematopoietic progenitor cells. Triple-color FACS analysis defined a new CD34+ CD19+ N-cadherin+ progenitor cell population. During further differentiation, however, N-cadherin expression is lost. Treatment of CD34+ progenitor cells with function-perturbing N-cadherin antibodies drastically diminished colony formation, indicating a direct involvement of N-cadherin in the differentiation program of early hematopoietic progenitors. N-cadherin can also mediate adhesive interactions within the bone marrow as demonstrated by inhibition of homotypic interactions of bone-marrow-derived cells with N-cadherin antibodies. Together, these data strongly suggest that N-cadherin is involved in the development and retention of early hematopoietic progenitors within the bone marrow microenvironment.

Original languageEnglish (US)
Pages (from-to)1567-1577
Number of pages11
JournalJournal of cell science
Volume114
Issue number8
StatePublished - May 22 2001

Fingerprint

Cadherins
Cell Differentiation
Bone Marrow Cells
Stem Cells
Bone Marrow
Hematopoietic System
Antibodies
Cell Adhesion Molecules
Hematopoietic Stem Cells
Mesenchymal Stromal Cells
Cell Adhesion
Adhesives
Muscle Cells
Embryonic Development
Fluorescent Antibody Technique
Endothelial Cells
Color
Western Blotting
Maintenance
Staining and Labeling

Keywords

  • Cadherin superfamily
  • Catenin
  • Cell adhesion
  • Cell differentiation
  • Hematopoiesis

ASJC Scopus subject areas

  • Cell Biology

Cite this

Puch, S., Armeanu, S., Kibler, C., Johnson, K. R., Müller, C. A., Wheelock, M. J., & Klein, G. (2001). N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation. Journal of cell science, 114(8), 1567-1577.

N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation. / Puch, Sabine; Armeanu, Sorin; Kibler, Christine; Johnson, Keith R.; Müller, Claudia A.; Wheelock, Margaret J.; Klein, Gerd.

In: Journal of cell science, Vol. 114, No. 8, 22.05.2001, p. 1567-1577.

Research output: Contribution to journalArticle

Puch, S, Armeanu, S, Kibler, C, Johnson, KR, Müller, CA, Wheelock, MJ & Klein, G 2001, 'N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation', Journal of cell science, vol. 114, no. 8, pp. 1567-1577.
Puch, Sabine ; Armeanu, Sorin ; Kibler, Christine ; Johnson, Keith R. ; Müller, Claudia A. ; Wheelock, Margaret J. ; Klein, Gerd. / N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation. In: Journal of cell science. 2001 ; Vol. 114, No. 8. pp. 1567-1577.
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