Mzm1 influences a labile pool of mitochondrial zinc important for respiratory function

Aaron Atkinson, Oleh Khalimonchuk, Pamela Smith, Hana Sabic, David Eide, Dennis R. Winge

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Zinc is essential for function of mitochondria as a cofactor for several matrix zinc metalloproteins. We demonstrate that a labile cationic zinc component of low molecular mass exists in the yeast mitochondrial matrix. This zinc pool is homeostatically regulated in response to the cellular zinc status. This pool of zinc is functionally important because matrix targeting of a cytosolic zinc-binding protein reduces the level of labile zinc and interferes with mitochondrial respiratory function. We identified a series of proteins that modulate the matrix zinc pool, one of which is a novel conserved mitochondrial protein designated Mzm1. Mutant mzm1Δ cells have reduced total and labile mitochondrial zinc, and these cells are hypersensitive to perturbations of the labile pool. In addition, mzm1Δ cells have a destabilized cytochrome c reductase (Complex III) without any effects on Complexes IV or V. Thus, we have established that a link exists between Complex III integrity and the labile mitochondrial zinc pool.

Original languageEnglish (US)
Pages (from-to)19450-19459
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number25
DOIs
StatePublished - Jun 18 2010

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Zinc
Electron Transport Complex III
Metalloproteins
Cytochrome Reductases
Mitochondria
Mitochondrial Proteins
Molecular mass
Cytochromes c
Yeast
Oxidoreductases
Yeasts

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mzm1 influences a labile pool of mitochondrial zinc important for respiratory function. / Atkinson, Aaron; Khalimonchuk, Oleh; Smith, Pamela; Sabic, Hana; Eide, David; Winge, Dennis R.

In: Journal of Biological Chemistry, Vol. 285, No. 25, 18.06.2010, p. 19450-19459.

Research output: Contribution to journalArticle

Atkinson, Aaron ; Khalimonchuk, Oleh ; Smith, Pamela ; Sabic, Hana ; Eide, David ; Winge, Dennis R. / Mzm1 influences a labile pool of mitochondrial zinc important for respiratory function. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 25. pp. 19450-19459.
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