Myotubularins, PtdIns5P, and ROS in ABA-mediated stomatal movements in dehydrated Arabidopsis seedlings

Akanksha Nagpal, Ammar Hassan, Ivan Ndamukong, Zoya Avramova, František Baluška

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Myotubularins (MTMs) are lipid phosphoinositide 3-phosphate phosphatases and the product of their enzyme activity - phosphoinositide 5-phosphate (PtdIns5P) - functions as a signalling molecule in pathways involved in membrane dynamics and cell signalling. Two Arabidopsis genes, AtMTM1 and AtMTM2, encode enzymatically active phosphatases but although AtMTM1 deficiency results in increased tolerance to dehydration stress and a decrease in cellular PtdIns5P, the role of AtMTM2 is less clear, as it does not contribute to the PtdIns5P pool upon dehydration stress. Here we analysed the involvement of AtMTM1, AtMTM2 and PtdIns5P in the response of Arabidopsis seedlings to dehydration stress/ABA, and found that both AtMTM1 and AtMTM2 were involved but affected oppositely stomata movement and the accumulation of reactive oxygen species (ROS, e.g. H2O2). Acting as a secondary messenger in the ABA-induced ROS production in guard cells, PtdIns5P emerges as an evolutionarily conserved signalling molecule that calibrates cellular ROS under stress. We propose the biological relevance of the counteracting AtMTM1 and AtMTM2 activities is to balance the ABA-induced ROS accumulation and cellular homeostasis under dehydration stress.

Original languageEnglish (US)
Pages (from-to)259-266
Number of pages8
JournalFunctional Plant Biology
Volume45
Issue number1-2
DOIs
StatePublished - Jan 1 2018

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stomatal movement
desiccation (plant physiology)
Arabidopsis
seedlings
phosphates
guard cells
stomata
reactive oxygen species
homeostasis
enzyme activity
lipids
genes
cells

Keywords

  • PI5P
  • drought stress
  • guard cells
  • stomata

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Myotubularins, PtdIns5P, and ROS in ABA-mediated stomatal movements in dehydrated Arabidopsis seedlings. / Nagpal, Akanksha; Hassan, Ammar; Ndamukong, Ivan; Avramova, Zoya; Baluška, František.

In: Functional Plant Biology, Vol. 45, No. 1-2, 01.01.2018, p. 259-266.

Research output: Contribution to journalArticle

Nagpal, Akanksha ; Hassan, Ammar ; Ndamukong, Ivan ; Avramova, Zoya ; Baluška, František. / Myotubularins, PtdIns5P, and ROS in ABA-mediated stomatal movements in dehydrated Arabidopsis seedlings. In: Functional Plant Biology. 2018 ; Vol. 45, No. 1-2. pp. 259-266.
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