Myogenic gene expression at rest and after a bout of resistance exercise in young (18-30 yr) and old (80-89 yr) women

Ulrika Raue, Dustin R Slivka, Bozena Jemiolo, Chris Hollon, Scott Trappe

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

The purpose of this study was to investigate mRNA expression of several key skeletal muscle myogenic controllers; myogenic differentiation factor (MyoD), muscle regulatory factor 4 (MRF4), myogenic factor 5 (Myf5), myogenin, myostatin, and myocyte enhancer factor 2 (MEF2) at rest and 4 h after a single bout of resistance exercise (RE) in young and old women. Eight young women (YW; 23 ± 2 yr, 67 ± 5 kg) and six old women (OW; 85 ± 1 yr, 67 ± 4 kg) performed 3 sets of 10 repetitions of bilateral knee extensions at 70% of one repetition maximum. Muscle biopsies were taken from the vastus lateralis before and 4 h after RE. Using real-time RT PCR, mRNA from the muscle samples was amplified and normalized to GAPDH. At rest, OW expressed higher (P < 0.05) levels of MyoD, MRF4, Myf5, myogenin, and myostatin compared with YW. In response to RE, there was a main time effect (P < 0.05) for the YW and OW combined in the upregulation of MyoD (2.0-fold) and MRF4 (1.4-fold) and in the downregulation of myostatin (2.2-fold). There was a trend (P = 0.08) for time X age interaction in MRF4. These data show that old women express higher myogenic mRNA levels at rest. The higher resting myogenic mRNA levels in old women may reflect an attempt to preserve muscle mass and function. When challenged with RE, old women appear to respond in a similar manner as young women.

Original languageEnglish (US)
Pages (from-to)53-59
Number of pages7
JournalJournal of Applied Physiology
Volume101
Issue number1
DOIs
StatePublished - Jul 17 2006

Fingerprint

Exercise
Gene Expression
Myostatin
Myogenic Regulatory Factor 5
Myogenin
Messenger RNA
Muscles
MEF2 Transcription Factors
Quadriceps Muscle
Real-Time Polymerase Chain Reaction
Knee
Skeletal Muscle
Up-Regulation
Down-Regulation
Biopsy
myogenic factor 6

Keywords

  • Muscle regulatory factors
  • Real-time reverse transcriptase-polymerase chain reaction
  • Skeletal muscle
  • mRNA

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Myogenic gene expression at rest and after a bout of resistance exercise in young (18-30 yr) and old (80-89 yr) women. / Raue, Ulrika; Slivka, Dustin R; Jemiolo, Bozena; Hollon, Chris; Trappe, Scott.

In: Journal of Applied Physiology, Vol. 101, No. 1, 17.07.2006, p. 53-59.

Research output: Contribution to journalArticle

@article{1b4399216695476b81f5659465cae2a5,
title = "Myogenic gene expression at rest and after a bout of resistance exercise in young (18-30 yr) and old (80-89 yr) women",
abstract = "The purpose of this study was to investigate mRNA expression of several key skeletal muscle myogenic controllers; myogenic differentiation factor (MyoD), muscle regulatory factor 4 (MRF4), myogenic factor 5 (Myf5), myogenin, myostatin, and myocyte enhancer factor 2 (MEF2) at rest and 4 h after a single bout of resistance exercise (RE) in young and old women. Eight young women (YW; 23 ± 2 yr, 67 ± 5 kg) and six old women (OW; 85 ± 1 yr, 67 ± 4 kg) performed 3 sets of 10 repetitions of bilateral knee extensions at 70{\%} of one repetition maximum. Muscle biopsies were taken from the vastus lateralis before and 4 h after RE. Using real-time RT PCR, mRNA from the muscle samples was amplified and normalized to GAPDH. At rest, OW expressed higher (P < 0.05) levels of MyoD, MRF4, Myf5, myogenin, and myostatin compared with YW. In response to RE, there was a main time effect (P < 0.05) for the YW and OW combined in the upregulation of MyoD (2.0-fold) and MRF4 (1.4-fold) and in the downregulation of myostatin (2.2-fold). There was a trend (P = 0.08) for time X age interaction in MRF4. These data show that old women express higher myogenic mRNA levels at rest. The higher resting myogenic mRNA levels in old women may reflect an attempt to preserve muscle mass and function. When challenged with RE, old women appear to respond in a similar manner as young women.",
keywords = "Muscle regulatory factors, Real-time reverse transcriptase-polymerase chain reaction, Skeletal muscle, mRNA",
author = "Ulrika Raue and Slivka, {Dustin R} and Bozena Jemiolo and Chris Hollon and Scott Trappe",
year = "2006",
month = "7",
day = "17",
doi = "10.1152/japplphysiol.01616.2005",
language = "English (US)",
volume = "101",
pages = "53--59",
journal = "Journal of Applied Physiology",
issn = "0161-7567",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Myogenic gene expression at rest and after a bout of resistance exercise in young (18-30 yr) and old (80-89 yr) women

AU - Raue, Ulrika

AU - Slivka, Dustin R

AU - Jemiolo, Bozena

AU - Hollon, Chris

AU - Trappe, Scott

PY - 2006/7/17

Y1 - 2006/7/17

N2 - The purpose of this study was to investigate mRNA expression of several key skeletal muscle myogenic controllers; myogenic differentiation factor (MyoD), muscle regulatory factor 4 (MRF4), myogenic factor 5 (Myf5), myogenin, myostatin, and myocyte enhancer factor 2 (MEF2) at rest and 4 h after a single bout of resistance exercise (RE) in young and old women. Eight young women (YW; 23 ± 2 yr, 67 ± 5 kg) and six old women (OW; 85 ± 1 yr, 67 ± 4 kg) performed 3 sets of 10 repetitions of bilateral knee extensions at 70% of one repetition maximum. Muscle biopsies were taken from the vastus lateralis before and 4 h after RE. Using real-time RT PCR, mRNA from the muscle samples was amplified and normalized to GAPDH. At rest, OW expressed higher (P < 0.05) levels of MyoD, MRF4, Myf5, myogenin, and myostatin compared with YW. In response to RE, there was a main time effect (P < 0.05) for the YW and OW combined in the upregulation of MyoD (2.0-fold) and MRF4 (1.4-fold) and in the downregulation of myostatin (2.2-fold). There was a trend (P = 0.08) for time X age interaction in MRF4. These data show that old women express higher myogenic mRNA levels at rest. The higher resting myogenic mRNA levels in old women may reflect an attempt to preserve muscle mass and function. When challenged with RE, old women appear to respond in a similar manner as young women.

AB - The purpose of this study was to investigate mRNA expression of several key skeletal muscle myogenic controllers; myogenic differentiation factor (MyoD), muscle regulatory factor 4 (MRF4), myogenic factor 5 (Myf5), myogenin, myostatin, and myocyte enhancer factor 2 (MEF2) at rest and 4 h after a single bout of resistance exercise (RE) in young and old women. Eight young women (YW; 23 ± 2 yr, 67 ± 5 kg) and six old women (OW; 85 ± 1 yr, 67 ± 4 kg) performed 3 sets of 10 repetitions of bilateral knee extensions at 70% of one repetition maximum. Muscle biopsies were taken from the vastus lateralis before and 4 h after RE. Using real-time RT PCR, mRNA from the muscle samples was amplified and normalized to GAPDH. At rest, OW expressed higher (P < 0.05) levels of MyoD, MRF4, Myf5, myogenin, and myostatin compared with YW. In response to RE, there was a main time effect (P < 0.05) for the YW and OW combined in the upregulation of MyoD (2.0-fold) and MRF4 (1.4-fold) and in the downregulation of myostatin (2.2-fold). There was a trend (P = 0.08) for time X age interaction in MRF4. These data show that old women express higher myogenic mRNA levels at rest. The higher resting myogenic mRNA levels in old women may reflect an attempt to preserve muscle mass and function. When challenged with RE, old women appear to respond in a similar manner as young women.

KW - Muscle regulatory factors

KW - Real-time reverse transcriptase-polymerase chain reaction

KW - Skeletal muscle

KW - mRNA

UR - http://www.scopus.com/inward/record.url?scp=33745813868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745813868&partnerID=8YFLogxK

U2 - 10.1152/japplphysiol.01616.2005

DO - 10.1152/japplphysiol.01616.2005

M3 - Article

VL - 101

SP - 53

EP - 59

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 0161-7567

IS - 1

ER -