Myocardial apoptosis after cardioplegic arrest in the neonatal lamb

James M. Hammel, Christopher A. Caldarone, Timothy L. Van Natta, Li Xing Wang, Karl F. Welke, Weigen Li, Scott Niles, Elisa Barner, Thomas D. Scholz, Douglas M. Behrendt, Jeffrey L. Segar, Carl L. Backer, Bradley S. Allen, Pedro L. Del Nido, Frank L. Hanley

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: Myocardial apoptosis is observed after various cardiac injuries and is also a normal part of fetal cardiac development and early postnatal maturation. Cardioplegic arrest and reperfusion result in ischemic injury and oxidative stress, known triggers of apoptosis. Because the neonatal heart is in a proapoptotic state, we hypothesize that apoptosis is triggered after cardioplegic arrest in neonatal myocardium. Methods: We started neonatal lambs (6-8 days old, n = 5) on cardiopulmonary bypass and administered cold crystalloid cardioplegia at 20-minute intervals. Total crossclamp time was 70 minutes, and bypass time was 90 minutes. After a six-hour recovery period, the hearts were excised and examined by using TdT-mediated dUTP nick-end labeling; radiolabeled DNA electrophoresis; fluorimetric caspase 3, 8, and 9 activity assay; mRNA microarray; and Western immunoblotting. Control lambs were anesthetized but did not undergo operation (n = 5) or were started on cardiopulmonary bypass for 90 minutes but not arrested (n = 5). Results: Lambs subjected to cardioplegia had 5-fold more TdT-mediated dUTP nick-end labeling-positive nuclei compared with that seen in unoperated control animals (P = .007) and bypass-only control animals (P = .008). DNA laddering was present in all postcardioplegia hearts but absent among control hearts. Bad and Bcl-X mRNA transcription increased significantly. Caspase 3, 8, and 9 activities were slightly greater than those seen in control animals, but the differences were not significant. No change was detected in Bcl-2, Bax, or Bcl-xL proteins. Conclusions: In a clinically relevant model of neonatal cardioplegic arrest, increased apoptotic cell death is present 6 hours after reperfusion, and both proapoptotic and antiapoptotic responses are triggered. The clinical implications of apoptosis after cardioplegic arrest remain undetermined.

Original languageEnglish (US)
Pages (from-to)1268-1273
Number of pages6
JournalJournal of Thoracic and Cardiovascular Surgery
Volume125
Issue number6
DOIs
StatePublished - Jun 1 2003

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Apoptosis
Induced Heart Arrest
Caspase 9
Caspase 8
Cardiopulmonary Bypass
Caspase 3
Reperfusion
Messenger RNA
DNA
Wounds and Injuries
Fetal Development
Electrophoresis
Myocardium
Oxidative Stress
Cell Death
Western Blotting
Proteins
crystalloid solutions

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Hammel, J. M., Caldarone, C. A., Van Natta, T. L., Wang, L. X., Welke, K. F., Li, W., ... Hanley, F. L. (2003). Myocardial apoptosis after cardioplegic arrest in the neonatal lamb. Journal of Thoracic and Cardiovascular Surgery, 125(6), 1268-1273. https://doi.org/10.1016/S0022-5223(02)73238-8

Myocardial apoptosis after cardioplegic arrest in the neonatal lamb. / Hammel, James M.; Caldarone, Christopher A.; Van Natta, Timothy L.; Wang, Li Xing; Welke, Karl F.; Li, Weigen; Niles, Scott; Barner, Elisa; Scholz, Thomas D.; Behrendt, Douglas M.; Segar, Jeffrey L.; Backer, Carl L.; Allen, Bradley S.; Del Nido, Pedro L.; Hanley, Frank L.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 125, No. 6, 01.06.2003, p. 1268-1273.

Research output: Contribution to journalArticle

Hammel, JM, Caldarone, CA, Van Natta, TL, Wang, LX, Welke, KF, Li, W, Niles, S, Barner, E, Scholz, TD, Behrendt, DM, Segar, JL, Backer, CL, Allen, BS, Del Nido, PL & Hanley, FL 2003, 'Myocardial apoptosis after cardioplegic arrest in the neonatal lamb', Journal of Thoracic and Cardiovascular Surgery, vol. 125, no. 6, pp. 1268-1273. https://doi.org/10.1016/S0022-5223(02)73238-8
Hammel, James M. ; Caldarone, Christopher A. ; Van Natta, Timothy L. ; Wang, Li Xing ; Welke, Karl F. ; Li, Weigen ; Niles, Scott ; Barner, Elisa ; Scholz, Thomas D. ; Behrendt, Douglas M. ; Segar, Jeffrey L. ; Backer, Carl L. ; Allen, Bradley S. ; Del Nido, Pedro L. ; Hanley, Frank L. / Myocardial apoptosis after cardioplegic arrest in the neonatal lamb. In: Journal of Thoracic and Cardiovascular Surgery. 2003 ; Vol. 125, No. 6. pp. 1268-1273.
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T1 - Myocardial apoptosis after cardioplegic arrest in the neonatal lamb

AU - Hammel, James M.

AU - Caldarone, Christopher A.

AU - Van Natta, Timothy L.

AU - Wang, Li Xing

AU - Welke, Karl F.

AU - Li, Weigen

AU - Niles, Scott

AU - Barner, Elisa

AU - Scholz, Thomas D.

AU - Behrendt, Douglas M.

AU - Segar, Jeffrey L.

AU - Backer, Carl L.

AU - Allen, Bradley S.

AU - Del Nido, Pedro L.

AU - Hanley, Frank L.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Objective: Myocardial apoptosis is observed after various cardiac injuries and is also a normal part of fetal cardiac development and early postnatal maturation. Cardioplegic arrest and reperfusion result in ischemic injury and oxidative stress, known triggers of apoptosis. Because the neonatal heart is in a proapoptotic state, we hypothesize that apoptosis is triggered after cardioplegic arrest in neonatal myocardium. Methods: We started neonatal lambs (6-8 days old, n = 5) on cardiopulmonary bypass and administered cold crystalloid cardioplegia at 20-minute intervals. Total crossclamp time was 70 minutes, and bypass time was 90 minutes. After a six-hour recovery period, the hearts were excised and examined by using TdT-mediated dUTP nick-end labeling; radiolabeled DNA electrophoresis; fluorimetric caspase 3, 8, and 9 activity assay; mRNA microarray; and Western immunoblotting. Control lambs were anesthetized but did not undergo operation (n = 5) or were started on cardiopulmonary bypass for 90 minutes but not arrested (n = 5). Results: Lambs subjected to cardioplegia had 5-fold more TdT-mediated dUTP nick-end labeling-positive nuclei compared with that seen in unoperated control animals (P = .007) and bypass-only control animals (P = .008). DNA laddering was present in all postcardioplegia hearts but absent among control hearts. Bad and Bcl-X mRNA transcription increased significantly. Caspase 3, 8, and 9 activities were slightly greater than those seen in control animals, but the differences were not significant. No change was detected in Bcl-2, Bax, or Bcl-xL proteins. Conclusions: In a clinically relevant model of neonatal cardioplegic arrest, increased apoptotic cell death is present 6 hours after reperfusion, and both proapoptotic and antiapoptotic responses are triggered. The clinical implications of apoptosis after cardioplegic arrest remain undetermined.

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