Mycobacterium talmoniae sp. Nov., a slowly growing mycobacterium isolated from human respiratory samples

Rebecca M. Davidson, Mary Ann DeGroote, Jamie L. Marola, Sarah Buss, Victoria Jones, Michael R. McNeil, Alison Gail Freifeld, L. Elaine Epperson, Nabeeh A. Hasan, Mary Jackson, Peter Charles Iwen, Max Salfinger, Michael Strong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7-10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 % and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 %), but the ANI between the genomes was 81.5 %, below the suggested cut-off for differentiating two species (95 %). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus. The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812 T (=ATCC BAA-2683 T =DSM 46873 T ).

Original languageEnglish (US)
Article number001998
Pages (from-to)2640-2645
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number8
DOIs
StatePublished - Jan 1 2017

Fingerprint

Mycobacterium
Mycobacterium avium
genome
Mycobacterium simiae
Mycobacterium abscessus
sampling
Genome
nucleotides
nucleotide sequences
Actinobacteria
acids
Nucleotides
Mycolic Acids
desorption
gene
acid
ionization
internal transcribed spacers
lasers
ribosomal RNA

Keywords

  • Genomics
  • Mycobacteria
  • Respiratory sample

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Davidson, R. M., DeGroote, M. A., Marola, J. L., Buss, S., Jones, V., McNeil, M. R., ... Strong, M. (2017). Mycobacterium talmoniae sp. Nov., a slowly growing mycobacterium isolated from human respiratory samples. International Journal of Systematic and Evolutionary Microbiology, 67(8), 2640-2645. [001998]. https://doi.org/10.1099/ijsem.0.001998

Mycobacterium talmoniae sp. Nov., a slowly growing mycobacterium isolated from human respiratory samples. / Davidson, Rebecca M.; DeGroote, Mary Ann; Marola, Jamie L.; Buss, Sarah; Jones, Victoria; McNeil, Michael R.; Freifeld, Alison Gail; Epperson, L. Elaine; Hasan, Nabeeh A.; Jackson, Mary; Iwen, Peter Charles; Salfinger, Max; Strong, Michael.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 8, 001998, 01.01.2017, p. 2640-2645.

Research output: Contribution to journalArticle

Davidson, RM, DeGroote, MA, Marola, JL, Buss, S, Jones, V, McNeil, MR, Freifeld, AG, Epperson, LE, Hasan, NA, Jackson, M, Iwen, PC, Salfinger, M & Strong, M 2017, 'Mycobacterium talmoniae sp. Nov., a slowly growing mycobacterium isolated from human respiratory samples', International Journal of Systematic and Evolutionary Microbiology, vol. 67, no. 8, 001998, pp. 2640-2645. https://doi.org/10.1099/ijsem.0.001998
Davidson, Rebecca M. ; DeGroote, Mary Ann ; Marola, Jamie L. ; Buss, Sarah ; Jones, Victoria ; McNeil, Michael R. ; Freifeld, Alison Gail ; Epperson, L. Elaine ; Hasan, Nabeeh A. ; Jackson, Mary ; Iwen, Peter Charles ; Salfinger, Max ; Strong, Michael. / Mycobacterium talmoniae sp. Nov., a slowly growing mycobacterium isolated from human respiratory samples. In: International Journal of Systematic and Evolutionary Microbiology. 2017 ; Vol. 67, No. 8. pp. 2640-2645.
@article{eaeed491fe7548ea8f61507b22335532,
title = "Mycobacterium talmoniae sp. Nov., a slowly growing mycobacterium isolated from human respiratory samples",
abstract = "A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7-10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 {\%} and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 {\%}), but the ANI between the genomes was 81.5 {\%}, below the suggested cut-off for differentiating two species (95 {\%}). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus. The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812 T (=ATCC BAA-2683 T =DSM 46873 T ).",
keywords = "Genomics, Mycobacteria, Respiratory sample",
author = "Davidson, {Rebecca M.} and DeGroote, {Mary Ann} and Marola, {Jamie L.} and Sarah Buss and Victoria Jones and McNeil, {Michael R.} and Freifeld, {Alison Gail} and Epperson, {L. Elaine} and Hasan, {Nabeeh A.} and Mary Jackson and Iwen, {Peter Charles} and Max Salfinger and Michael Strong",
year = "2017",
month = "1",
day = "1",
doi = "10.1099/ijsem.0.001998",
language = "English (US)",
volume = "67",
pages = "2640--2645",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "8",

}

TY - JOUR

T1 - Mycobacterium talmoniae sp. Nov., a slowly growing mycobacterium isolated from human respiratory samples

AU - Davidson, Rebecca M.

AU - DeGroote, Mary Ann

AU - Marola, Jamie L.

AU - Buss, Sarah

AU - Jones, Victoria

AU - McNeil, Michael R.

AU - Freifeld, Alison Gail

AU - Epperson, L. Elaine

AU - Hasan, Nabeeh A.

AU - Jackson, Mary

AU - Iwen, Peter Charles

AU - Salfinger, Max

AU - Strong, Michael

PY - 2017/1/1

Y1 - 2017/1/1

N2 - A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7-10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 % and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 %), but the ANI between the genomes was 81.5 %, below the suggested cut-off for differentiating two species (95 %). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus. The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812 T (=ATCC BAA-2683 T =DSM 46873 T ).

AB - A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7-10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 % and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 %), but the ANI between the genomes was 81.5 %, below the suggested cut-off for differentiating two species (95 %). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus. The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812 T (=ATCC BAA-2683 T =DSM 46873 T ).

KW - Genomics

KW - Mycobacteria

KW - Respiratory sample

UR - http://www.scopus.com/inward/record.url?scp=85029505116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029505116&partnerID=8YFLogxK

U2 - 10.1099/ijsem.0.001998

DO - 10.1099/ijsem.0.001998

M3 - Article

VL - 67

SP - 2640

EP - 2645

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 8

M1 - 001998

ER -