Mycobacterium nebraskense sp. nov., a novel slowly growing scotochromogenic species

Amr M. Mohamed, Peter Charles Iwen, Stefano Tarantolo, Steven Heye Hinrichs

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The characterization of a novel slowly growing, scotochromogenic Mycobacterium species is reported. This previously undescribed mycobacterial species was isolated from five different patients with symptomatic pulmonary infections. All isolates were acid-fast-positive and the mycolic acid profiles were unique and supported placement into the genus Mycobacterium. Phenotypic characteristics of each strain included optimal growth after 3 weeks at a temperature range of 30-35 °C, yellow pigmentation after incubation in the dark and production of a heat-stable catalase. The 16S rRNA gene and internal transcribed spacer 1 sequences were identical for all five strains, but distinct from all known mycobacterial species. Phylogenetic analysis based on the 16S rRNA gene sequence placed the novel species within the slowly growing mycobacteria group in close proximity to Mycobacterium malmoense, Mycobacterium avium, Mycobacterium kansasii and Mycobacterium scrofulaceum. These data support the conclusion that the related five described organisms represent a novel Mycobacterium species, for which the name Mycobacterium nebraskense sp. nov. is proposed, with the type strain UNMC-MY1349T (=ATCC BAA-837T = DSM 44803T).

Original languageEnglish (US)
Pages (from-to)2057-2060
Number of pages4
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume54
Issue number6
DOIs
StatePublished - Nov 1 2004

Fingerprint

Mycobacterium
new species
Mycobacterium malmoense
Mycobacterium scrofulaceum
rRNA Genes
Mycobacterium kansasii
ribosomal RNA
Mycolic Acids
Mycobacterium avium
gene
acids
acid
heat production
Thermogenesis
pigmentation
internal transcribed spacers
Pigmentation
catalase
Catalase
incubation

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Mycobacterium nebraskense sp. nov., a novel slowly growing scotochromogenic species. / Mohamed, Amr M.; Iwen, Peter Charles; Tarantolo, Stefano; Hinrichs, Steven Heye.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 54, No. 6, 01.11.2004, p. 2057-2060.

Research output: Contribution to journalArticle

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