Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype

R. Allende, G. F. Kutish, W. Laegreid, Z. Lu, T. L. Lewis, D. L. Rock, J. Friesen, J. A. Galeota, Alan R Doster, F. A. Osorio

Research output: Contribution to journalArticle

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Abstract

Although live-attenuated vaccines have been used for some time to control clinical symptoms of the porcine reproductive and respiratory syndrome (PRRS), the molecular bases for the attenuated phenotype remain unclear. We had previously determined the genomic sequence of the pathogenic PRRSV 16244B. Limited comparisons of the structural protein coding sequence of an attenuated vaccine strain have shown 98% homology to the pathogenic 16244B. Here we have confirmed the attenuated phenotype and determined the genomic sequence of that attenuated PRRSV vaccine and compared it to its parental VR-2332 and the 16244B strains. The attenuated vaccine sequence was colinear with that of the strain 16244B sequence containing no gaps and 212 substitutions over 15,374 determined nucleotide sequence. We identified nine amino acid changes distributed in Nsp1β, Nsp2, Nsp10, ORF2, ORF3, ORF5 and ORF6. These changes may provide the molecular bases for the observed attenuated phenotype.

Original languageEnglish (US)
Pages (from-to)1149-1161
Number of pages13
JournalArchives of Virology
Volume145
Issue number6
DOIs
StatePublished - Jul 22 2000

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Porcine respiratory and reproductive syndrome virus
Attenuated Vaccines
Genome
Phenotype
Mutation
Porcine Reproductive and Respiratory Syndrome
Amino Acids
Proteins

ASJC Scopus subject areas

  • Virology

Cite this

Allende, R., Kutish, G. F., Laegreid, W., Lu, Z., Lewis, T. L., Rock, D. L., ... Osorio, F. A. (2000). Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype. Archives of Virology, 145(6), 1149-1161. https://doi.org/10.1007/s007050070115

Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype. / Allende, R.; Kutish, G. F.; Laegreid, W.; Lu, Z.; Lewis, T. L.; Rock, D. L.; Friesen, J.; Galeota, J. A.; Doster, Alan R; Osorio, F. A.

In: Archives of Virology, Vol. 145, No. 6, 22.07.2000, p. 1149-1161.

Research output: Contribution to journalArticle

Allende, R, Kutish, GF, Laegreid, W, Lu, Z, Lewis, TL, Rock, DL, Friesen, J, Galeota, JA, Doster, AR & Osorio, FA 2000, 'Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype', Archives of Virology, vol. 145, no. 6, pp. 1149-1161. https://doi.org/10.1007/s007050070115
Allende, R. ; Kutish, G. F. ; Laegreid, W. ; Lu, Z. ; Lewis, T. L. ; Rock, D. L. ; Friesen, J. ; Galeota, J. A. ; Doster, Alan R ; Osorio, F. A. / Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype. In: Archives of Virology. 2000 ; Vol. 145, No. 6. pp. 1149-1161.
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