Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7

Gaylen A. Uhlich, James Keen, Robert O. Elder

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.

Original languageEnglish (US)
Pages (from-to)2367-2370
Number of pages4
JournalApplied and environmental microbiology
Volume67
Issue number5
DOIs
StatePublished - May 1 2001

Fingerprint

Escherichia coli O157
phenotype
mutation
promoter regions
Phenotype
Congo Red
Mutation
Operon
Base Pairing
dye binding
Disease Outbreaks
Organism Cloning
dye
Coloring Agents
Republic of the Congo
operon
substrate
molecular cloning
cloning
fibre

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7. / Uhlich, Gaylen A.; Keen, James; Elder, Robert O.

In: Applied and environmental microbiology, Vol. 67, No. 5, 01.05.2001, p. 2367-2370.

Research output: Contribution to journalArticle

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