Mutational analysis of the role of HPr in Listeria monocytogenes

Douglas P. Christensen, Andrew K Benson, Robert W Hutkins

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12 Citations (Scopus)

Abstract

The regulatory role of HPr, a protein of the phosphotransferase system (PTS), was investigated in Listeria monocytogenes. By constructing mutations in the conserved histidine 15 and serine 46 residues of HPr, we were able to examine how HPr regulates PTS activity. The results indicated that histidine 15 was phosphorylated in a phosphoenolpyruvate (PEP) dependent manner and was essential for PTS activity. Serine 46 was phosphorylated in an ATP-dependent manner by a membrane-associated kinase. ATP-dependent phosphorylation of serine 46 was significantly enhanced in the presence of fructose 1,6- diphosphate and resulted in a reduction of PTS activity. The presence of a charge at position 15 did not inhibit ATP-dependent phosphorylation of serine 46, a finding unique to gram-positive PEP-dependent PTSs studied to this point. Finally, HPr phosphorylated at serine 46 does not appear to possess self-phosphatase activity, suggesting a specific phosphatase protein may be essential for the recycling of HPr to its active form.

Original languageEnglish (US)
Pages (from-to)2112-2115
Number of pages4
JournalApplied and environmental microbiology
Volume65
Issue number5
StatePublished - May 1 1999

Fingerprint

Listeria monocytogenes
phosphatase
serine
Serine
phosphotransferases (kinases)
Phosphotransferases
protein
Phosphoenolpyruvate
Adenosine Triphosphate
mutation
histidine
recycling
Histidine
membrane
phosphorylation
Phosphorylation
Phosphoprotein Phosphatases
Recycling
Phosphoric Monoester Hydrolases
fructose

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Mutational analysis of the role of HPr in Listeria monocytogenes. / Christensen, Douglas P.; Benson, Andrew K; Hutkins, Robert W.

In: Applied and environmental microbiology, Vol. 65, No. 5, 01.05.1999, p. 2112-2115.

Research output: Contribution to journalArticle

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