Mutagenicity in V79 cells of N-nitrosobis(2-hydroxypropyl)amine and N-nitrosobis(2-oxopropyl)amine activated by tissues from hamsters fed low and high fat diets

Terence Lawson

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3 Citations (Scopus)

Abstract

Hepatocytes and pancreas duct tissues from male Syrian hamsters fed high-fat (HFD) and low-fat (LFD) diets were used to activate N-nitrosobis(2-hydroxypropyl)amine (BHP) and N-nitrosobis(2-oxopropyl)amine (BOP) In the V79 cell mutagenicity assay. V79 DNA alkylation by BHP and BOP was also measured. There was a 3.5-fold increase in BHP mutagenicity but only a 1.4-fold increase in BOP mutagenicity when hepatocytes from HFD-fed hamsters were used over the mutagenicity when hepatocytes from LFD-fed hamsters were used. When pancreas duct tissue was the activating system there was a 2-fold increase in BHP and BOP mutagenicity. O6-Methylguanine levels in V79 DNA rose 4-fold when hepatocytes from HFD-fed hamsters were used to activate BOP but they declined when BHP was the alkylating agent.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalCarcinogenesis
Volume13
Issue number2
DOIs
StatePublished - Feb 1 1992

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diisopropanolnitrosamine
nitrosobis(2-oxopropyl)amine
Fat-Restricted Diet
High Fat Diet
Cricetinae
Hepatocytes
Pancreas
Alkylating Agents
DNA
Mesocricetus
Alkylation
Fats

ASJC Scopus subject areas

  • Cancer Research

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Mutagenicity in V79 cells of N-nitrosobis(2-hydroxypropyl)amine and N-nitrosobis(2-oxopropyl)amine activated by tissues from hamsters fed low and high fat diets. / Lawson, Terence.

In: Carcinogenesis, Vol. 13, No. 2, 01.02.1992, p. 223-226.

Research output: Contribution to journalArticle

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