Murine monoclonal antibodies against Escherichia coli O4 lipopolysaccharide and H5 flagellin

M. Rivera-Betancourt, J. E. Keen

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Abstract

Two murine monoclonal antibodies (MAb), 2C5-F10 and 8D1-H10, reactive with Escherichia coli O4 and H5 antigens, respectively, were generated and characterized. Enzyme immunoassays and immunoblots demonstrated that MAb 2C5-F10 reacted specifically with lipopolysaccharide O antigen of E. coli O4 isolates, while MAb 8D1-H10 reacted with E. coli strains expressing H5 flagella.

Original languageEnglish (US)
Pages (from-to)3409-3413
Number of pages5
JournalJournal of clinical microbiology
Volume39
Issue number9
DOIs
Publication statusPublished - Sep 21 2001

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ASJC Scopus subject areas

  • Microbiology (medical)

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