Muramic acid, endotoxin, 3-hydroxy fatty acids, and ergosterol content explain monocyte and epithelial cell inflammatory responses to agricultural dusts

Jill A Poole, Gregory P. Dooley, Rena Saito, Angela M. Burrell, Kristina L Bailey, Debra Romberger, John Mehaffy, Stephen J. Reynolds

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

In agricultural and other environments, inhalation of airborne microorganisms is linked to respiratory disease development. Bacterial endotoxins, peptidoglycans, and fungi are potential causative agents, but relative microbial characterization and inflammatory comparisons amongst agricultural dusts are not well described. The aim of this study was to determine the distribution of microbial endotoxin, 3-hydroxy fatty acids (3-OHFA), muramic acid, and ergosterol and evaluate inflammatory responses in human monocytes and bronchial epithelial cells with various dust samples. Settled surface dust was obtained from five environments: swine facility, dairy barn, grain elevator, domestic home (no pets), and domestic home with dog. Endotoxin concentration was determined by recombinant factor C (rFC). 3-OHFA, muramic acid, and ergosterol were measured using gas chromatography-mass spectrometry. Dust-induced inflammatory cytokine secretion in human monocytes and bronchial epithelial cells was evaluated. Endotoxin-independent dust-induced inflammatory responses were evaluated. Endotoxin and 3-OHFA levels were highest in agricultural dusts. Muramic acid, endotoxin, 3-OHFA, and ergosterol were detected in dusts samples. Muramic acid was highest in animal farming dusts. Ergosterol was most significant in grain elevator dust. Agricultural dusts induced monocyte tumor necrosis factor (TNF)α , interleukin (IL)-6, IL-8, and epithelial cell IL-6 and IL-8 secretion. Monocyte and epithelial IL-6 and IL-8 secretion was not dependent on endotoxin. House dust(s) induced monocyte TNFα, IL-6, and IL-8 secretion. Swine facility dust generally produced elevated responses compared to other dusts. Agricultural dusts are complex with significant microbial component contribution. Large animal farming dust(s)-induced inflammation is not entirely dependent on endotoxin. Addition of muramic acid to endotoxin in large animal farming environment monitoring is warranted.

Original languageEnglish (US)
Pages (from-to)684-700
Number of pages17
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume73
Issue number10
DOIs
StatePublished - Jan 1 2010

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Muramic Acids
Ergosterol
Hydroxy Acids
Dust
Endotoxins
Monocytes
Fatty Acids
Epithelial Cells
Interleukin-8
Grain elevators
Interleukin-6
Agriculture
Elevators and Escalators
Animals
Swine
Tumor Necrosis Factor-alpha
Pulmonary diseases

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Muramic acid, endotoxin, 3-hydroxy fatty acids, and ergosterol content explain monocyte and epithelial cell inflammatory responses to agricultural dusts. / Poole, Jill A; Dooley, Gregory P.; Saito, Rena; Burrell, Angela M.; Bailey, Kristina L; Romberger, Debra; Mehaffy, John; Reynolds, Stephen J.

In: Journal of Toxicology and Environmental Health - Part A: Current Issues, Vol. 73, No. 10, 01.01.2010, p. 684-700.

Research output: Contribution to journalArticle

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