Multiple forms of brain adenylate cyclase: Stimulation by Mn2+

Daniel F. Malamuda, Concetta C. DiRusso, June R. Aprille

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mn2+-stimulated adenylate cyclase (ATP pyrophosphate-lyase-(cyclizing), EC 4.6.1.1) activity in detergent solubilized preparations from mouse brain. While NaF-stimulated activity was decreased by both solubilization and storage at 0-4°C, the ability of the enzyme to be stimulated by Mn2+ was maintained for up to one week. By including Mn+ in the assay of adenylate cyclase in gel fractions after isoelectric focusing, two distinct peaks of enzyme activity (pI1 = 5.8, pI2 = 6.4) were detected, suggesting the existence of more than one type of catalytic subunit in mouse brain cell membranes.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalBBA - Enzymology
Volume485
Issue number1
DOIs
StatePublished - Nov 23 1977

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Adenylyl Cyclases
Brain
Isoelectric Focusing
Enzymes
Detergents
Catalytic Domain
Gels
Cell Membrane

ASJC Scopus subject areas

  • Medicine(all)

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Multiple forms of brain adenylate cyclase : Stimulation by Mn2+. / Malamuda, Daniel F.; DiRusso, Concetta C.; Aprille, June R.

In: BBA - Enzymology, Vol. 485, No. 1, 23.11.1977, p. 243-247.

Research output: Contribution to journalArticle

Malamuda, Daniel F. ; DiRusso, Concetta C. ; Aprille, June R. / Multiple forms of brain adenylate cyclase : Stimulation by Mn2+. In: BBA - Enzymology. 1977 ; Vol. 485, No. 1. pp. 243-247.
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