Mucosal vaccination by adenoviruses displaying reovirus sigma 1

Eric A. Weaver, Zenaido T. Camacho, Matthew L. Hillestad, Catherine M. Crosby, Mallory A. Turner, Adam J. Guenzel, Hind J. Fadel, George T. Mercier, Michael A. Barry

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We developed adenovirus serotype 5 (Ad5) vectors displaying the sigma 1 protein from reovirus as mucosal vaccines. Ad5-sigma retargets to JAM-1 and sialic acid, but has 40-fold reduced gene delivery when compared to Ad5. While weaker at transduction, Ad5-sigma generates stronger T cell responses than Ad5 when used for mucosal immunization. In this work, new Ad5-fiber-sigma vectors were generated by varying the number of fiber β-spiral shaft repeats (R) between the fiber tail and sigma. Increasing chimera length led to decreasing insertion of these proteinsAd5 virions. Ad-R3 and R14 vectors effectively targeted JAM-1 in vitro while R20 did not. When wereused to immunize mice by the intranasal route, Ad5-R3-sigma produced higher serum and vaginal antibody responses than Ad5. These data suggest optimized Ad-sigma vectors may be useful vectors for mucosal vaccination.

Original languageEnglish (US)
Pages (from-to)60-66
Number of pages7
JournalVirology
Volume482
DOIs
StatePublished - Aug 1 2015

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Mammalian Orthoreovirus
Adenoviridae
Vaccination
Serogroup
N-Acetylneuraminic Acid
Virion
Antibody Formation
Tail
Immunization
Vaccines
T-Lymphocytes

Keywords

  • Adenovirus
  • Immunization
  • Mucosal
  • Reovirus
  • Sigma 1

ASJC Scopus subject areas

  • Virology

Cite this

Weaver, E. A., Camacho, Z. T., Hillestad, M. L., Crosby, C. M., Turner, M. A., Guenzel, A. J., ... Barry, M. A. (2015). Mucosal vaccination by adenoviruses displaying reovirus sigma 1. Virology, 482, 60-66. https://doi.org/10.1016/j.virol.2015.02.050

Mucosal vaccination by adenoviruses displaying reovirus sigma 1. / Weaver, Eric A.; Camacho, Zenaido T.; Hillestad, Matthew L.; Crosby, Catherine M.; Turner, Mallory A.; Guenzel, Adam J.; Fadel, Hind J.; Mercier, George T.; Barry, Michael A.

In: Virology, Vol. 482, 01.08.2015, p. 60-66.

Research output: Contribution to journalArticle

Weaver, EA, Camacho, ZT, Hillestad, ML, Crosby, CM, Turner, MA, Guenzel, AJ, Fadel, HJ, Mercier, GT & Barry, MA 2015, 'Mucosal vaccination by adenoviruses displaying reovirus sigma 1', Virology, vol. 482, pp. 60-66. https://doi.org/10.1016/j.virol.2015.02.050
Weaver EA, Camacho ZT, Hillestad ML, Crosby CM, Turner MA, Guenzel AJ et al. Mucosal vaccination by adenoviruses displaying reovirus sigma 1. Virology. 2015 Aug 1;482:60-66. https://doi.org/10.1016/j.virol.2015.02.050
Weaver, Eric A. ; Camacho, Zenaido T. ; Hillestad, Matthew L. ; Crosby, Catherine M. ; Turner, Mallory A. ; Guenzel, Adam J. ; Fadel, Hind J. ; Mercier, George T. ; Barry, Michael A. / Mucosal vaccination by adenoviruses displaying reovirus sigma 1. In: Virology. 2015 ; Vol. 482. pp. 60-66.
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