Mucosal gene expression profiles following the colonization of immunocompetent defined-flora C3H mice with Helicobacter bilis: a prelude to typhlocolitis

Zhiping Liu, Abigail L. Henderson, Dan Nettleton, Jennifer H. Wilson-Welder, Jesse M. Hostetter, Amanda Ramer-Tait, Albert E. Jergens, Michael J. Wannemuehler

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An aberrant immune response to the commensal microbiota is widely hypothesized to contribute to the development of inflammatory bowel disease. Helicobacter bilis colonization of defined-flora mice has been shown to trigger host immune responses to the commensal flora. However, the magnitude of the effects on mucosal homeostasis following colonization with H. bilis has not been determined. Using microarray analysis, differential gene expression within the cecal mucosa was assessed at 15, 30, or 45 days following H. bilis colonization using Affymetrix Genechips. H. bilis colonization induced marked upregulation of genes associated with protein metabolism, immune responses, and downregulation of genes associated with fatty acid metabolism and detoxification which peaked at 15 days postinfection. A set of genes associated with glycoprotein synthesis and detoxification including Fut2, B3galt5, Ceacam12, Cyp4b1, and Ugt8a were uniquely identified and found to be similarly expressed following the induction of typhlocolitis by dextran sodium sulfate or Brachyspira hyodysenteriae. This study provides preliminary evidence as to the types of factors or changes in the intestinal mucosa that potentially predispose the host to the development of typhlocolitis.

Original languageEnglish (US)
Pages (from-to)374-383
Number of pages10
JournalMicrobes and Infection
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2009

Fingerprint

Helicobacter
Inbred C3H Mouse
Transcriptome
Brachyspira hyodysenteriae
Genes
Dextran Sulfate
Microbiota
Intestinal Mucosa
Microarray Analysis
Inflammatory Bowel Diseases
Glycoproteins
Mucous Membrane
Homeostasis
Up-Regulation
Fatty Acids
Down-Regulation
Gene Expression
Proteins

Keywords

  • Gene expression
  • Helicobacter bilis
  • Mucosa
  • Susceptibility
  • Typhlocolitis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Mucosal gene expression profiles following the colonization of immunocompetent defined-flora C3H mice with Helicobacter bilis : a prelude to typhlocolitis. / Liu, Zhiping; Henderson, Abigail L.; Nettleton, Dan; Wilson-Welder, Jennifer H.; Hostetter, Jesse M.; Ramer-Tait, Amanda; Jergens, Albert E.; Wannemuehler, Michael J.

In: Microbes and Infection, Vol. 11, No. 3, 01.03.2009, p. 374-383.

Research output: Contribution to journalArticle

Liu, Zhiping ; Henderson, Abigail L. ; Nettleton, Dan ; Wilson-Welder, Jennifer H. ; Hostetter, Jesse M. ; Ramer-Tait, Amanda ; Jergens, Albert E. ; Wannemuehler, Michael J. / Mucosal gene expression profiles following the colonization of immunocompetent defined-flora C3H mice with Helicobacter bilis : a prelude to typhlocolitis. In: Microbes and Infection. 2009 ; Vol. 11, No. 3. pp. 374-383.
@article{f2c64e20de774d568ec352e36fe69dd3,
title = "Mucosal gene expression profiles following the colonization of immunocompetent defined-flora C3H mice with Helicobacter bilis: a prelude to typhlocolitis",
abstract = "An aberrant immune response to the commensal microbiota is widely hypothesized to contribute to the development of inflammatory bowel disease. Helicobacter bilis colonization of defined-flora mice has been shown to trigger host immune responses to the commensal flora. However, the magnitude of the effects on mucosal homeostasis following colonization with H. bilis has not been determined. Using microarray analysis, differential gene expression within the cecal mucosa was assessed at 15, 30, or 45 days following H. bilis colonization using Affymetrix Genechips. H. bilis colonization induced marked upregulation of genes associated with protein metabolism, immune responses, and downregulation of genes associated with fatty acid metabolism and detoxification which peaked at 15 days postinfection. A set of genes associated with glycoprotein synthesis and detoxification including Fut2, B3galt5, Ceacam12, Cyp4b1, and Ugt8a were uniquely identified and found to be similarly expressed following the induction of typhlocolitis by dextran sodium sulfate or Brachyspira hyodysenteriae. This study provides preliminary evidence as to the types of factors or changes in the intestinal mucosa that potentially predispose the host to the development of typhlocolitis.",
keywords = "Gene expression, Helicobacter bilis, Mucosa, Susceptibility, Typhlocolitis",
author = "Zhiping Liu and Henderson, {Abigail L.} and Dan Nettleton and Wilson-Welder, {Jennifer H.} and Hostetter, {Jesse M.} and Amanda Ramer-Tait and Jergens, {Albert E.} and Wannemuehler, {Michael J.}",
year = "2009",
month = "3",
day = "1",
doi = "10.1016/j.micinf.2008.12.013",
language = "English (US)",
volume = "11",
pages = "374--383",
journal = "Microbes and Infection",
issn = "1286-4579",
publisher = "Elsevier Masson SAS",
number = "3",

}

TY - JOUR

T1 - Mucosal gene expression profiles following the colonization of immunocompetent defined-flora C3H mice with Helicobacter bilis

T2 - a prelude to typhlocolitis

AU - Liu, Zhiping

AU - Henderson, Abigail L.

AU - Nettleton, Dan

AU - Wilson-Welder, Jennifer H.

AU - Hostetter, Jesse M.

AU - Ramer-Tait, Amanda

AU - Jergens, Albert E.

AU - Wannemuehler, Michael J.

PY - 2009/3/1

Y1 - 2009/3/1

N2 - An aberrant immune response to the commensal microbiota is widely hypothesized to contribute to the development of inflammatory bowel disease. Helicobacter bilis colonization of defined-flora mice has been shown to trigger host immune responses to the commensal flora. However, the magnitude of the effects on mucosal homeostasis following colonization with H. bilis has not been determined. Using microarray analysis, differential gene expression within the cecal mucosa was assessed at 15, 30, or 45 days following H. bilis colonization using Affymetrix Genechips. H. bilis colonization induced marked upregulation of genes associated with protein metabolism, immune responses, and downregulation of genes associated with fatty acid metabolism and detoxification which peaked at 15 days postinfection. A set of genes associated with glycoprotein synthesis and detoxification including Fut2, B3galt5, Ceacam12, Cyp4b1, and Ugt8a were uniquely identified and found to be similarly expressed following the induction of typhlocolitis by dextran sodium sulfate or Brachyspira hyodysenteriae. This study provides preliminary evidence as to the types of factors or changes in the intestinal mucosa that potentially predispose the host to the development of typhlocolitis.

AB - An aberrant immune response to the commensal microbiota is widely hypothesized to contribute to the development of inflammatory bowel disease. Helicobacter bilis colonization of defined-flora mice has been shown to trigger host immune responses to the commensal flora. However, the magnitude of the effects on mucosal homeostasis following colonization with H. bilis has not been determined. Using microarray analysis, differential gene expression within the cecal mucosa was assessed at 15, 30, or 45 days following H. bilis colonization using Affymetrix Genechips. H. bilis colonization induced marked upregulation of genes associated with protein metabolism, immune responses, and downregulation of genes associated with fatty acid metabolism and detoxification which peaked at 15 days postinfection. A set of genes associated with glycoprotein synthesis and detoxification including Fut2, B3galt5, Ceacam12, Cyp4b1, and Ugt8a were uniquely identified and found to be similarly expressed following the induction of typhlocolitis by dextran sodium sulfate or Brachyspira hyodysenteriae. This study provides preliminary evidence as to the types of factors or changes in the intestinal mucosa that potentially predispose the host to the development of typhlocolitis.

KW - Gene expression

KW - Helicobacter bilis

KW - Mucosa

KW - Susceptibility

KW - Typhlocolitis

UR - http://www.scopus.com/inward/record.url?scp=62649153261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62649153261&partnerID=8YFLogxK

U2 - 10.1016/j.micinf.2008.12.013

DO - 10.1016/j.micinf.2008.12.013

M3 - Article

C2 - 19397886

AN - SCOPUS:62649153261

VL - 11

SP - 374

EP - 383

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

IS - 3

ER -