Mucins and associated O-glycans based immunoprofile for stratification of colorectal polyps

Clinical implication for improved colon surveillance

Shiv Ram Krishn, Sukwinder Kaur, Yuri M. Sheinin, Lynette M Smith, Shailendra K. Gautam, Asish Patel, Maneesh Jain, Vasthala Juvvigunta, Priya Pai, Audrey J Lazenby, Hemant K. Roy, Surinder Kumar Batra

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Sessile serrated adenoma/polyps (SSA/P) are premalignant lesions of colorectal cancer that are difficult to distinguish histologically from hyperplastic polyps (HP) of minimal to no malignant potential. Specific markers for differentiating SSA/P from HP can aid clinicians for optimizing colon surveillance intervals. The present study investigates the potential of mucins and associated O-glycans to distinguish SSA/P from HP. Expression of colonic mucins (MUC1, MUC4, MUC17, MUC2, and MUC5AC) and O-glycans [Sialyl LewisA (CA19-9) and Tn/Sialyl-Tn on MUC1] were analyzed in HP (n=33), SSA/P (n=39), and tubular adenoma (TA) (n=36) samples by immunohistochemistry. A significantly reduced expression of MUC4 (p=0.0066), elevated expression of MUC17 (p=0.0002), and MUC5AC (p < 0.0001) was observed in SSA/P cases in comparison to HP cases. Interestingly, significantly higher number of SSA/P cases (p < 0.0001) exhibited MUC5AC expression in the goblet cells as well as filled the crypt lumen compared to only goblet cells in majority of the HP cases. Improved diagnostic potential was revealed by multivariate logistic regression analysis where combinatorial panel of MUC5AC/MUC17 discriminated SSA/P from HP (SN/SP=85/82%). Finally, the decision tree model based marker panel (CA19-9/ MUC17/MUC5AC) predicted HP, SSA/P and TA with SN/SP of 58%/95%, 79%/90% and 97%/83%, respectively. Overall, the mucin and associated O-glycan based panel defined in the present study could aid in discriminating SSA/P from HP to devise better colon surveillance strategies.

Original languageEnglish (US)
Pages (from-to)7025-7038
Number of pages14
JournalOncotarget
Volume8
Issue number4
DOIs
StatePublished - 2017

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Mucins
Polyps
Polysaccharides
Colon
Adenoma
Goblet Cells
Decision Trees

Keywords

  • Hyperplastic polyps
  • Mucins
  • O-glycans
  • Sessile serrated adenoma/polyps
  • Tubular adenomas

ASJC Scopus subject areas

  • Oncology

Cite this

Mucins and associated O-glycans based immunoprofile for stratification of colorectal polyps : Clinical implication for improved colon surveillance. / Krishn, Shiv Ram; Kaur, Sukwinder; Sheinin, Yuri M.; Smith, Lynette M; Gautam, Shailendra K.; Patel, Asish; Jain, Maneesh; Juvvigunta, Vasthala; Pai, Priya; Lazenby, Audrey J; Roy, Hemant K.; Batra, Surinder Kumar.

In: Oncotarget, Vol. 8, No. 4, 2017, p. 7025-7038.

Research output: Contribution to journalArticle

Krishn, Shiv Ram ; Kaur, Sukwinder ; Sheinin, Yuri M. ; Smith, Lynette M ; Gautam, Shailendra K. ; Patel, Asish ; Jain, Maneesh ; Juvvigunta, Vasthala ; Pai, Priya ; Lazenby, Audrey J ; Roy, Hemant K. ; Batra, Surinder Kumar. / Mucins and associated O-glycans based immunoprofile for stratification of colorectal polyps : Clinical implication for improved colon surveillance. In: Oncotarget. 2017 ; Vol. 8, No. 4. pp. 7025-7038.
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abstract = "Sessile serrated adenoma/polyps (SSA/P) are premalignant lesions of colorectal cancer that are difficult to distinguish histologically from hyperplastic polyps (HP) of minimal to no malignant potential. Specific markers for differentiating SSA/P from HP can aid clinicians for optimizing colon surveillance intervals. The present study investigates the potential of mucins and associated O-glycans to distinguish SSA/P from HP. Expression of colonic mucins (MUC1, MUC4, MUC17, MUC2, and MUC5AC) and O-glycans [Sialyl LewisA (CA19-9) and Tn/Sialyl-Tn on MUC1] were analyzed in HP (n=33), SSA/P (n=39), and tubular adenoma (TA) (n=36) samples by immunohistochemistry. A significantly reduced expression of MUC4 (p=0.0066), elevated expression of MUC17 (p=0.0002), and MUC5AC (p < 0.0001) was observed in SSA/P cases in comparison to HP cases. Interestingly, significantly higher number of SSA/P cases (p < 0.0001) exhibited MUC5AC expression in the goblet cells as well as filled the crypt lumen compared to only goblet cells in majority of the HP cases. Improved diagnostic potential was revealed by multivariate logistic regression analysis where combinatorial panel of MUC5AC/MUC17 discriminated SSA/P from HP (SN/SP=85/82{\%}). Finally, the decision tree model based marker panel (CA19-9/ MUC17/MUC5AC) predicted HP, SSA/P and TA with SN/SP of 58{\%}/95{\%}, 79{\%}/90{\%} and 97{\%}/83{\%}, respectively. Overall, the mucin and associated O-glycan based panel defined in the present study could aid in discriminating SSA/P from HP to devise better colon surveillance strategies.",
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AU - Sheinin, Yuri M.

AU - Smith, Lynette M

AU - Gautam, Shailendra K.

AU - Patel, Asish

AU - Jain, Maneesh

AU - Juvvigunta, Vasthala

AU - Pai, Priya

AU - Lazenby, Audrey J

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AU - Batra, Surinder Kumar

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