Mucins and associated glycan signatures in colon adenoma-carcinoma sequence: Prospective pathological implication(s) for early diagnosis of colon cancer

Shiv Ram Krishn, Sukwinder Kaur, Lynette M Smith, Sonny L. Johansson, Maneesh Jain, Asish Patel, Shailendra K. Gautam, Michael A Hollingsworth, Ulla Mandel, Henrik Clausen, Wing Cheong Lo, Wai Tong Louis Fan, Upender Manne, Surinder Kumar Batra

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Development of biomarkers that detect early stage resectable premalignant lesions of colon can provide critical aid in the prevention of colorectal cancer. Recent lines of evidence suggest the utility of mucin expression to predict malignant transformation of colon pre-neoplastic lesions. In this study, we investigated the combined expression of multiple mucins and mucin-associated glycans during the adenoma-carcinoma sequence of colon cancer progression. Further, we evaluated their applicability as markers for differentiating adenomas/adenocarcinomas from hyperplastic polyps. Immunohistochemical analyses performed on colon disease tissue microarrays revealed downregulation of MUC2 and MUC4 expression (p < 0.0001) while MUC1 and MUC5AC expressions were upregulated (p = 0.01) during adenoma-adenocarcinoma progression. Expression of MUC17 was downregulated in inflamed tissues compared to normal tissues, but its increased expression differentiated adenomas (p = 0.0028) and adenocarcinomas (p = 0.025) from inflammation. Glycan epitope-Tn/STn on MUC1 showed higher expression in hyperplastic polyps (p = 0.023), adenomas (p = 0.042) and adenocarcinomas (p = 0.0096) compared to normal tissues. Multivariate regression analyses indicated that a combination of MUC2, MUC5AC, and MUC17 could effectively discriminate adenoma-adenocarcinoma from hyperplastic polyps. Altogether, a combined analysis of altered mucins and mucin-associated glycans is a useful approach to distinguish premalignant/malignant lesions of colon from benign polyps.

Original languageEnglish (US)
Pages (from-to)304-314
Number of pages11
JournalCancer Letters
Volume374
Issue number2
DOIs
StatePublished - May 1 2016

Fingerprint

Mucins
Early Detection of Cancer
Adenoma
Colonic Neoplasms
Polysaccharides
Colon
Polyps
Adenocarcinoma
Carcinoma
Down-Regulation
Epitopes
Colorectal Neoplasms
Multivariate Analysis
Biomarkers
Regression Analysis
Inflammation

Keywords

  • Adenoma
  • Colonic mucin
  • Colorectal cancer
  • Glycan
  • Hyperplastic polyp

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Mucins and associated glycan signatures in colon adenoma-carcinoma sequence : Prospective pathological implication(s) for early diagnosis of colon cancer. / Krishn, Shiv Ram; Kaur, Sukwinder; Smith, Lynette M; Johansson, Sonny L.; Jain, Maneesh; Patel, Asish; Gautam, Shailendra K.; Hollingsworth, Michael A; Mandel, Ulla; Clausen, Henrik; Lo, Wing Cheong; Fan, Wai Tong Louis; Manne, Upender; Batra, Surinder Kumar.

In: Cancer Letters, Vol. 374, No. 2, 01.05.2016, p. 304-314.

Research output: Contribution to journalArticle

Krishn, Shiv Ram ; Kaur, Sukwinder ; Smith, Lynette M ; Johansson, Sonny L. ; Jain, Maneesh ; Patel, Asish ; Gautam, Shailendra K. ; Hollingsworth, Michael A ; Mandel, Ulla ; Clausen, Henrik ; Lo, Wing Cheong ; Fan, Wai Tong Louis ; Manne, Upender ; Batra, Surinder Kumar. / Mucins and associated glycan signatures in colon adenoma-carcinoma sequence : Prospective pathological implication(s) for early diagnosis of colon cancer. In: Cancer Letters. 2016 ; Vol. 374, No. 2. pp. 304-314.
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