Mucin Expression in Endoscopic Ultrasound-Guided Fine-Needle Aspiration Specimens Is a Useful Prognostic Factor in Pancreatic Ductal Adenocarcinoma

Michiyo Higashi, Seiya Yokoyama, Takafumi Yamamoto, Yuko Goto, Ikumi Kitazono, Tsubasa Hiraki, Hiroki Taguchi, Shinichi Hashimoto, Yoshihiko Fukukura, Chihaya Koriyama, Yuko Mataki, Kosei Maemura, Hiroyuki Shinchi, Maneesh Jain, Surinder Kumar Batra, Suguru Yonezawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objectives: The aim of this study was to further examine the utility of mucin (MUC) expression profiles as prognostic factors in pancreatic ductal adenocarcinoma (PDAC). Methods: Mucin expression was examined by immunohistochemistry analysis in endoscopic ultrasound-guided fine-needle aspiration specimens obtained from 114 patients with PDAC. The rate of expression of each MUC was compared with clinicopathologic features. Results: The expression rates of MUCs in cancer lesions were MUC1, 87.7%; MUC2, 0.8%; MUC4, 93.0%; MUC5AC, 78.9%; MUC6, 24.6%; and MUC16, 67.5%. MUC1 and MUC4 were positive, and MUC2 was negative in most PDACs. Patients with advanced stage of PDAC with MUC5AC expression had a significantly better outcome than those who were MUC5AC-negative (P = 0.002). With increasing clinical stage, total MUC6 expression decreased (P for trend = 0.001) and MUC16 cytoplasmic expression increased (P for trend = 0.02). The prognosis of patients with MUC16 cytoplasmic expression was significantly poorer than those without this expression. Multivariate survival analysis revealed that MUC16 cytoplasmic expression was a significant independent predictor of a poor prognosis after adjusting for the effects of other prognostic factors (P = 0.002). Conclusions: Mucin expression profiles in ultrasound-guided fine-needle aspiration specimens have excellent diagnostic utility and are useful predictors of outcome in patients with PDAC.

Original languageEnglish (US)
Pages (from-to)728-734
Number of pages7
JournalPancreas
Volume44
Issue number5
DOIs
StatePublished - Jul 25 2015

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Endoscopic Ultrasound-Guided Fine Needle Aspiration
Mucins
Adenocarcinoma
Survival Analysis
Fine Needle Biopsy
Multivariate Analysis
Immunohistochemistry
Neoplasms

Keywords

  • FNA
  • MUC
  • carcinoma
  • pancreas
  • prognosis

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Cite this

Mucin Expression in Endoscopic Ultrasound-Guided Fine-Needle Aspiration Specimens Is a Useful Prognostic Factor in Pancreatic Ductal Adenocarcinoma. / Higashi, Michiyo; Yokoyama, Seiya; Yamamoto, Takafumi; Goto, Yuko; Kitazono, Ikumi; Hiraki, Tsubasa; Taguchi, Hiroki; Hashimoto, Shinichi; Fukukura, Yoshihiko; Koriyama, Chihaya; Mataki, Yuko; Maemura, Kosei; Shinchi, Hiroyuki; Jain, Maneesh; Batra, Surinder Kumar; Yonezawa, Suguru.

In: Pancreas, Vol. 44, No. 5, 25.07.2015, p. 728-734.

Research output: Contribution to journalArticle

Higashi, M, Yokoyama, S, Yamamoto, T, Goto, Y, Kitazono, I, Hiraki, T, Taguchi, H, Hashimoto, S, Fukukura, Y, Koriyama, C, Mataki, Y, Maemura, K, Shinchi, H, Jain, M, Batra, SK & Yonezawa, S 2015, 'Mucin Expression in Endoscopic Ultrasound-Guided Fine-Needle Aspiration Specimens Is a Useful Prognostic Factor in Pancreatic Ductal Adenocarcinoma', Pancreas, vol. 44, no. 5, pp. 728-734. https://doi.org/10.1097/MPA.0000000000000362
Higashi, Michiyo ; Yokoyama, Seiya ; Yamamoto, Takafumi ; Goto, Yuko ; Kitazono, Ikumi ; Hiraki, Tsubasa ; Taguchi, Hiroki ; Hashimoto, Shinichi ; Fukukura, Yoshihiko ; Koriyama, Chihaya ; Mataki, Yuko ; Maemura, Kosei ; Shinchi, Hiroyuki ; Jain, Maneesh ; Batra, Surinder Kumar ; Yonezawa, Suguru. / Mucin Expression in Endoscopic Ultrasound-Guided Fine-Needle Aspiration Specimens Is a Useful Prognostic Factor in Pancreatic Ductal Adenocarcinoma. In: Pancreas. 2015 ; Vol. 44, No. 5. pp. 728-734.
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title = "Mucin Expression in Endoscopic Ultrasound-Guided Fine-Needle Aspiration Specimens Is a Useful Prognostic Factor in Pancreatic Ductal Adenocarcinoma",
abstract = "Objectives: The aim of this study was to further examine the utility of mucin (MUC) expression profiles as prognostic factors in pancreatic ductal adenocarcinoma (PDAC). Methods: Mucin expression was examined by immunohistochemistry analysis in endoscopic ultrasound-guided fine-needle aspiration specimens obtained from 114 patients with PDAC. The rate of expression of each MUC was compared with clinicopathologic features. Results: The expression rates of MUCs in cancer lesions were MUC1, 87.7{\%}; MUC2, 0.8{\%}; MUC4, 93.0{\%}; MUC5AC, 78.9{\%}; MUC6, 24.6{\%}; and MUC16, 67.5{\%}. MUC1 and MUC4 were positive, and MUC2 was negative in most PDACs. Patients with advanced stage of PDAC with MUC5AC expression had a significantly better outcome than those who were MUC5AC-negative (P = 0.002). With increasing clinical stage, total MUC6 expression decreased (P for trend = 0.001) and MUC16 cytoplasmic expression increased (P for trend = 0.02). The prognosis of patients with MUC16 cytoplasmic expression was significantly poorer than those without this expression. Multivariate survival analysis revealed that MUC16 cytoplasmic expression was a significant independent predictor of a poor prognosis after adjusting for the effects of other prognostic factors (P = 0.002). Conclusions: Mucin expression profiles in ultrasound-guided fine-needle aspiration specimens have excellent diagnostic utility and are useful predictors of outcome in patients with PDAC.",
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T1 - Mucin Expression in Endoscopic Ultrasound-Guided Fine-Needle Aspiration Specimens Is a Useful Prognostic Factor in Pancreatic Ductal Adenocarcinoma

AU - Higashi, Michiyo

AU - Yokoyama, Seiya

AU - Yamamoto, Takafumi

AU - Goto, Yuko

AU - Kitazono, Ikumi

AU - Hiraki, Tsubasa

AU - Taguchi, Hiroki

AU - Hashimoto, Shinichi

AU - Fukukura, Yoshihiko

AU - Koriyama, Chihaya

AU - Mataki, Yuko

AU - Maemura, Kosei

AU - Shinchi, Hiroyuki

AU - Jain, Maneesh

AU - Batra, Surinder Kumar

AU - Yonezawa, Suguru

PY - 2015/7/25

Y1 - 2015/7/25

N2 - Objectives: The aim of this study was to further examine the utility of mucin (MUC) expression profiles as prognostic factors in pancreatic ductal adenocarcinoma (PDAC). Methods: Mucin expression was examined by immunohistochemistry analysis in endoscopic ultrasound-guided fine-needle aspiration specimens obtained from 114 patients with PDAC. The rate of expression of each MUC was compared with clinicopathologic features. Results: The expression rates of MUCs in cancer lesions were MUC1, 87.7%; MUC2, 0.8%; MUC4, 93.0%; MUC5AC, 78.9%; MUC6, 24.6%; and MUC16, 67.5%. MUC1 and MUC4 were positive, and MUC2 was negative in most PDACs. Patients with advanced stage of PDAC with MUC5AC expression had a significantly better outcome than those who were MUC5AC-negative (P = 0.002). With increasing clinical stage, total MUC6 expression decreased (P for trend = 0.001) and MUC16 cytoplasmic expression increased (P for trend = 0.02). The prognosis of patients with MUC16 cytoplasmic expression was significantly poorer than those without this expression. Multivariate survival analysis revealed that MUC16 cytoplasmic expression was a significant independent predictor of a poor prognosis after adjusting for the effects of other prognostic factors (P = 0.002). Conclusions: Mucin expression profiles in ultrasound-guided fine-needle aspiration specimens have excellent diagnostic utility and are useful predictors of outcome in patients with PDAC.

AB - Objectives: The aim of this study was to further examine the utility of mucin (MUC) expression profiles as prognostic factors in pancreatic ductal adenocarcinoma (PDAC). Methods: Mucin expression was examined by immunohistochemistry analysis in endoscopic ultrasound-guided fine-needle aspiration specimens obtained from 114 patients with PDAC. The rate of expression of each MUC was compared with clinicopathologic features. Results: The expression rates of MUCs in cancer lesions were MUC1, 87.7%; MUC2, 0.8%; MUC4, 93.0%; MUC5AC, 78.9%; MUC6, 24.6%; and MUC16, 67.5%. MUC1 and MUC4 were positive, and MUC2 was negative in most PDACs. Patients with advanced stage of PDAC with MUC5AC expression had a significantly better outcome than those who were MUC5AC-negative (P = 0.002). With increasing clinical stage, total MUC6 expression decreased (P for trend = 0.001) and MUC16 cytoplasmic expression increased (P for trend = 0.02). The prognosis of patients with MUC16 cytoplasmic expression was significantly poorer than those without this expression. Multivariate survival analysis revealed that MUC16 cytoplasmic expression was a significant independent predictor of a poor prognosis after adjusting for the effects of other prognostic factors (P = 0.002). Conclusions: Mucin expression profiles in ultrasound-guided fine-needle aspiration specimens have excellent diagnostic utility and are useful predictors of outcome in patients with PDAC.

KW - FNA

KW - MUC

KW - carcinoma

KW - pancreas

KW - prognosis

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DO - 10.1097/MPA.0000000000000362

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