MUC4 mucin expression in human pancreatic tumours is affected by organ environment

The possible role of TGFβ2

A. Choudhury, N. Moniaux, A. B. Ulrich, B. M. Schmied, J. Standop, P. M. Pour, S. J. Gendler, Michael A Hollingsworth, J. P. Aubert, Surinder Kumar Batra

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

MUC4 is highly expressed in human pancreatic tumours and pancreatic tumour cell lines, but is minimally or not expressed in normal pancreas or chronic pancreatitis. Here, we investigated the aberrant regulation of MUC4 expression in vivo using clonal human pancreatic tumour cells (CD18/HPAF) grown either orthotopically in the pancreas (OT) or ectopically in subcutaneous tissue (SC) in the nude mice. Histological examination of the OT and SC tumours showed moderately differentiated and anaplastic morphology, respectively. The OT tumour cells showed metastases to distant lymph nodes and faster tumour growth (P < 0.01) compared to the SC tumours. The MUC4 transcripts in OT tumours were very high compared to the undetectable levels in SC tumours. The SC tumour cells regained their ability to express MUC4 transcripts after in vitro culture. Immunohistochemical analysis using MUC4-specific polyclonal antiserum confirmed the results obtained by Northern blot analysis. Interestingly, the OT tumours showed expression of TGFβ2 compared to no expression in SC, suggesting a possible link between MUC4 and TGFβ2. The MUC4 expression, morphology, and metastasis of human pancreatic tumour cells are regulated by a local host microenvironment. TGFβ2 may serve as an interim regulator of this function.

Original languageEnglish (US)
Pages (from-to)657-664
Number of pages8
JournalBritish journal of cancer
Volume90
Issue number3
DOIs
StatePublished - Feb 9 2004

Fingerprint

Mucins
Subcutaneous Tissue
Neoplasms
Pancreas
Neoplasm Metastasis
Chronic Pancreatitis
Tumor Cell Line
Nude Mice
Northern Blotting
Immune Sera
Lymph Nodes

Keywords

  • MUC4
  • Mucin
  • Orthotopic environment
  • Pancreatic adenocarcinoma
  • TGFβ2

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

MUC4 mucin expression in human pancreatic tumours is affected by organ environment : The possible role of TGFβ2. / Choudhury, A.; Moniaux, N.; Ulrich, A. B.; Schmied, B. M.; Standop, J.; Pour, P. M.; Gendler, S. J.; Hollingsworth, Michael A; Aubert, J. P.; Batra, Surinder Kumar.

In: British journal of cancer, Vol. 90, No. 3, 09.02.2004, p. 657-664.

Research output: Contribution to journalArticle

Choudhury, A, Moniaux, N, Ulrich, AB, Schmied, BM, Standop, J, Pour, PM, Gendler, SJ, Hollingsworth, MA, Aubert, JP & Batra, SK 2004, 'MUC4 mucin expression in human pancreatic tumours is affected by organ environment: The possible role of TGFβ2', British journal of cancer, vol. 90, no. 3, pp. 657-664. https://doi.org/10.1038/sj.bjc.6601604
Choudhury, A. ; Moniaux, N. ; Ulrich, A. B. ; Schmied, B. M. ; Standop, J. ; Pour, P. M. ; Gendler, S. J. ; Hollingsworth, Michael A ; Aubert, J. P. ; Batra, Surinder Kumar. / MUC4 mucin expression in human pancreatic tumours is affected by organ environment : The possible role of TGFβ2. In: British journal of cancer. 2004 ; Vol. 90, No. 3. pp. 657-664.
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