MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer

W. R. Matull, F. Andreola, A. Loh, Z. Adiguzel, M. Deheragoda, U. Qureshi, Surinder Kumar Batra, D. M. Swallow, S. P. Pereira

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Alterations in epithelial mucin expression are associated with carcinogenesis, but there are few data in biliary tract cancer (BTC). In pancreatic malignancy, MUC4 is a diagnostic and prognostic tumour marker, whereas MUC5AC has been proposed as a sensitive serological marker for BTC. We assessed MUC4 and MUC5AC expression in (i) prospectively collected bile and serum specimens from 72 patients with biliary obstruction (39 BTC) by real-time reverse transcriptase-PCR (qPCR) and western blot analysis, and (ii) 79 archived biliary tissues (69 BTC) by immunohistochemistry. In bile, MUC4 protein was detected in 27% of BTC and 29% of primary sclerosing cholangitis (PSC) cases, but not in other benign and malignant biliary diseases (P<0.01 and P=0.06). qPCR revealed a 1.9-fold increased MUC4 mRNA expression in BTC patients' bile compared with benign disease. In archived tissues, MUC4 protein was detected in 37% of BTC but in none of the benign samples (P=0.03). In serum, MUC5AC was found exclusively in BTC and PSC sera (44% and 13%, respectively; P<0.001 for BTC vs non-BTC) and correlated negatively with BTC survival. Biliary MUC4 and serum MUC5AC are highly specific tumour-associated mucins that may be useful in the diagnosis and formulation of therapeutic strategies in BTC.

Original languageEnglish (US)
Pages (from-to)1675-1681
Number of pages7
JournalBritish journal of cancer
Volume98
Issue number10
DOIs
StatePublished - May 20 2008

Fingerprint

Biliary Tract Neoplasms
Mucins
Neoplasms
Bile
Sclerosing Cholangitis
Serum
Tumor Biomarkers
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Carcinogenesis
Proteins

Keywords

  • Biliary tract cancer
  • Cholangiocarcinoma
  • Mucin glycoprotein
  • Tumour marker

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Matull, W. R., Andreola, F., Loh, A., Adiguzel, Z., Deheragoda, M., Qureshi, U., ... Pereira, S. P. (2008). MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer. British journal of cancer, 98(10), 1675-1681. https://doi.org/10.1038/sj.bjc.6604364

MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer. / Matull, W. R.; Andreola, F.; Loh, A.; Adiguzel, Z.; Deheragoda, M.; Qureshi, U.; Batra, Surinder Kumar; Swallow, D. M.; Pereira, S. P.

In: British journal of cancer, Vol. 98, No. 10, 20.05.2008, p. 1675-1681.

Research output: Contribution to journalArticle

Matull, WR, Andreola, F, Loh, A, Adiguzel, Z, Deheragoda, M, Qureshi, U, Batra, SK, Swallow, DM & Pereira, SP 2008, 'MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer', British journal of cancer, vol. 98, no. 10, pp. 1675-1681. https://doi.org/10.1038/sj.bjc.6604364
Matull WR, Andreola F, Loh A, Adiguzel Z, Deheragoda M, Qureshi U et al. MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer. British journal of cancer. 2008 May 20;98(10):1675-1681. https://doi.org/10.1038/sj.bjc.6604364
Matull, W. R. ; Andreola, F. ; Loh, A. ; Adiguzel, Z. ; Deheragoda, M. ; Qureshi, U. ; Batra, Surinder Kumar ; Swallow, D. M. ; Pereira, S. P. / MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer. In: British journal of cancer. 2008 ; Vol. 98, No. 10. pp. 1675-1681.
@article{3deff194077443688d17e17fc46dabb9,
title = "MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer",
abstract = "Alterations in epithelial mucin expression are associated with carcinogenesis, but there are few data in biliary tract cancer (BTC). In pancreatic malignancy, MUC4 is a diagnostic and prognostic tumour marker, whereas MUC5AC has been proposed as a sensitive serological marker for BTC. We assessed MUC4 and MUC5AC expression in (i) prospectively collected bile and serum specimens from 72 patients with biliary obstruction (39 BTC) by real-time reverse transcriptase-PCR (qPCR) and western blot analysis, and (ii) 79 archived biliary tissues (69 BTC) by immunohistochemistry. In bile, MUC4 protein was detected in 27{\%} of BTC and 29{\%} of primary sclerosing cholangitis (PSC) cases, but not in other benign and malignant biliary diseases (P<0.01 and P=0.06). qPCR revealed a 1.9-fold increased MUC4 mRNA expression in BTC patients' bile compared with benign disease. In archived tissues, MUC4 protein was detected in 37{\%} of BTC but in none of the benign samples (P=0.03). In serum, MUC5AC was found exclusively in BTC and PSC sera (44{\%} and 13{\%}, respectively; P<0.001 for BTC vs non-BTC) and correlated negatively with BTC survival. Biliary MUC4 and serum MUC5AC are highly specific tumour-associated mucins that may be useful in the diagnosis and formulation of therapeutic strategies in BTC.",
keywords = "Biliary tract cancer, Cholangiocarcinoma, Mucin glycoprotein, Tumour marker",
author = "Matull, {W. R.} and F. Andreola and A. Loh and Z. Adiguzel and M. Deheragoda and U. Qureshi and Batra, {Surinder Kumar} and Swallow, {D. M.} and Pereira, {S. P.}",
year = "2008",
month = "5",
day = "20",
doi = "10.1038/sj.bjc.6604364",
language = "English (US)",
volume = "98",
pages = "1675--1681",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer

AU - Matull, W. R.

AU - Andreola, F.

AU - Loh, A.

AU - Adiguzel, Z.

AU - Deheragoda, M.

AU - Qureshi, U.

AU - Batra, Surinder Kumar

AU - Swallow, D. M.

AU - Pereira, S. P.

PY - 2008/5/20

Y1 - 2008/5/20

N2 - Alterations in epithelial mucin expression are associated with carcinogenesis, but there are few data in biliary tract cancer (BTC). In pancreatic malignancy, MUC4 is a diagnostic and prognostic tumour marker, whereas MUC5AC has been proposed as a sensitive serological marker for BTC. We assessed MUC4 and MUC5AC expression in (i) prospectively collected bile and serum specimens from 72 patients with biliary obstruction (39 BTC) by real-time reverse transcriptase-PCR (qPCR) and western blot analysis, and (ii) 79 archived biliary tissues (69 BTC) by immunohistochemistry. In bile, MUC4 protein was detected in 27% of BTC and 29% of primary sclerosing cholangitis (PSC) cases, but not in other benign and malignant biliary diseases (P<0.01 and P=0.06). qPCR revealed a 1.9-fold increased MUC4 mRNA expression in BTC patients' bile compared with benign disease. In archived tissues, MUC4 protein was detected in 37% of BTC but in none of the benign samples (P=0.03). In serum, MUC5AC was found exclusively in BTC and PSC sera (44% and 13%, respectively; P<0.001 for BTC vs non-BTC) and correlated negatively with BTC survival. Biliary MUC4 and serum MUC5AC are highly specific tumour-associated mucins that may be useful in the diagnosis and formulation of therapeutic strategies in BTC.

AB - Alterations in epithelial mucin expression are associated with carcinogenesis, but there are few data in biliary tract cancer (BTC). In pancreatic malignancy, MUC4 is a diagnostic and prognostic tumour marker, whereas MUC5AC has been proposed as a sensitive serological marker for BTC. We assessed MUC4 and MUC5AC expression in (i) prospectively collected bile and serum specimens from 72 patients with biliary obstruction (39 BTC) by real-time reverse transcriptase-PCR (qPCR) and western blot analysis, and (ii) 79 archived biliary tissues (69 BTC) by immunohistochemistry. In bile, MUC4 protein was detected in 27% of BTC and 29% of primary sclerosing cholangitis (PSC) cases, but not in other benign and malignant biliary diseases (P<0.01 and P=0.06). qPCR revealed a 1.9-fold increased MUC4 mRNA expression in BTC patients' bile compared with benign disease. In archived tissues, MUC4 protein was detected in 37% of BTC but in none of the benign samples (P=0.03). In serum, MUC5AC was found exclusively in BTC and PSC sera (44% and 13%, respectively; P<0.001 for BTC vs non-BTC) and correlated negatively with BTC survival. Biliary MUC4 and serum MUC5AC are highly specific tumour-associated mucins that may be useful in the diagnosis and formulation of therapeutic strategies in BTC.

KW - Biliary tract cancer

KW - Cholangiocarcinoma

KW - Mucin glycoprotein

KW - Tumour marker

UR - http://www.scopus.com/inward/record.url?scp=43649106488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43649106488&partnerID=8YFLogxK

U2 - 10.1038/sj.bjc.6604364

DO - 10.1038/sj.bjc.6604364

M3 - Article

C2 - 18475301

AN - SCOPUS:43649106488

VL - 98

SP - 1675

EP - 1681

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 10

ER -