MUC16

Molecular analysis and its functional implications in benign and malignant conditions

Dhanya Haridas, Moorthy Palanimuthu Ponnusamy, Seema Chugh, Imayavaramban Lakshmanan, Parthasarathy Seshacharyulu, Surinder Kumar Batra

Research output: Contribution to journalReview article

35 Citations (Scopus)

Abstract

MUC16 is a high-molecular-weight glycoprotein that is expressed by the various epithelial cell surfaces of the human body to protect the cell layer from a myriad of insults. It is the largest mucin known to date, with an ∼22,152 aa sequence. Structurally, MUC16 is characterized into 3 distinct domains: the amino terminal, the tandem repeat, and the carboxyl terminal domain, with each domain having unique attributes. The extracellular portion of MUC16 is shed into the bloodstream and serves as a biomarker for diagnosing and monitoring patients with cancer; however, its functional role in cancer is yet to be elucidated. Several factors contribute to this challenge, which include the large protein size; the extensive glycosylation that the protein undergoes, which confers functional heterogeneity; lack of specific antibodies that detect the unique domains of MUC16; and the existence of splicing variants. Despite these limitations, MUC16 has been established as a molecule of significant application in cancer. Hence, in this review, we discuss the various aspects of MUC16, which include its discovery, structure, and biological significance both in benign and malignant conditions with an attempt to dissect its functional relevance.

Original languageEnglish (US)
Pages (from-to)4183-4199
Number of pages17
JournalFASEB Journal
Volume28
Issue number10
DOIs
StatePublished - Oct 1 2014

Fingerprint

Glycosylation
Patient monitoring
Tandem Repeat Sequences
Biomarkers
Mucins
Neoplasms
Glycoproteins
Proteins
Terminal Repeat Sequences
Molecular weight
Cells
Physiologic Monitoring
Human Body
Molecules
Antibodies
Molecular Weight
Epithelial Cells

Keywords

  • Animal model
  • Cancer
  • Domain structure
  • MUC16

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

MUC16 : Molecular analysis and its functional implications in benign and malignant conditions. / Haridas, Dhanya; Palanimuthu Ponnusamy, Moorthy; Chugh, Seema; Lakshmanan, Imayavaramban; Seshacharyulu, Parthasarathy; Batra, Surinder Kumar.

In: FASEB Journal, Vol. 28, No. 10, 01.10.2014, p. 4183-4199.

Research output: Contribution to journalReview article

Haridas, Dhanya ; Palanimuthu Ponnusamy, Moorthy ; Chugh, Seema ; Lakshmanan, Imayavaramban ; Seshacharyulu, Parthasarathy ; Batra, Surinder Kumar. / MUC16 : Molecular analysis and its functional implications in benign and malignant conditions. In: FASEB Journal. 2014 ; Vol. 28, No. 10. pp. 4183-4199.
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