MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer

Nina V. Chaika, Teklab Gebregiworgis, Michelle E. Lewallen, Vinee Purohit, Prakash Radhakrishnan, Xiang Liu, Bo Zhang, Kamiya Mehla, Roger B. Brown, Thomas Caffrey, Fang Yu, Keith R Johnson, Robert Powers, Michael A Hollingsworth, Pankaj Singh

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate growth properties of cancer cells. MUC1 occupies the promoter elements of multiple genes directly involved in glucose metabolism and regulates their expression. Furthermore, MUC1 expression enhances glycolytic activity in pancreatic cancer cells. We also demonstrate that MUC1 expression enhances in vivo glucose uptake and expression of genes involved in glucose uptake and metabolism in orthotopic implantation models of pancreatic cancer. The MUC1 cytoplasmic tail is known to activate multiple signaling pathways through its interactions with several transcription factors/coregulators at the promoter elements of various genes. Our results indicate that MUC1 acts as a modulator of the hypoxic response in pancreatic cancer cells by regulating the expression/stability and activity of hypoxia-inducible factor-1α (HIF-1α). MUC1 physically interacts with HIF-1α and p300 and stabilizes the former at the protein level. By using a ChIP assay, we demonstrate that MUC1 facilitates recruitment of HIF-1α and p300 on glycolytic gene promoters in a hypoxia-dependent manner. Also, by metabolomic studies, we demonstrate that MUC1 regulates multiple metabolite intermediates in the glucose and amino acid metabolic pathways. Thus, our studies indicate that MUC1 acts as a master regulator of the metabolic program and facilitates metabolic alterations in the hypoxic environments that help tumor cells survive and proliferate under such conditions.

Original languageEnglish (US)
Pages (from-to)13787-13792
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number34
DOIs
StatePublished - Aug 21 2012

Fingerprint

Hypoxia-Inducible Factor 1
Mucins
Pancreatic Neoplasms
Glucose
Neoplasms
Genes
Metabolomics
Metabolic Networks and Pathways
Cell Survival
Proteins
Adenocarcinoma
Transcription Factors
Cell Proliferation
Gene Expression
Amino Acids
Growth

Keywords

  • 2-Ketoglutarate
  • Cancer metabolism
  • Glutamine accumulation
  • Pentose phosphate pathway

ASJC Scopus subject areas

  • General

Cite this

MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer. / Chaika, Nina V.; Gebregiworgis, Teklab; Lewallen, Michelle E.; Purohit, Vinee; Radhakrishnan, Prakash; Liu, Xiang; Zhang, Bo; Mehla, Kamiya; Brown, Roger B.; Caffrey, Thomas; Yu, Fang; Johnson, Keith R; Powers, Robert; Hollingsworth, Michael A; Singh, Pankaj.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 34, 21.08.2012, p. 13787-13792.

Research output: Contribution to journalArticle

Chaika, Nina V. ; Gebregiworgis, Teklab ; Lewallen, Michelle E. ; Purohit, Vinee ; Radhakrishnan, Prakash ; Liu, Xiang ; Zhang, Bo ; Mehla, Kamiya ; Brown, Roger B. ; Caffrey, Thomas ; Yu, Fang ; Johnson, Keith R ; Powers, Robert ; Hollingsworth, Michael A ; Singh, Pankaj. / MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 34. pp. 13787-13792.
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T1 - MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer

AU - Chaika, Nina V.

AU - Gebregiworgis, Teklab

AU - Lewallen, Michelle E.

AU - Purohit, Vinee

AU - Radhakrishnan, Prakash

AU - Liu, Xiang

AU - Zhang, Bo

AU - Mehla, Kamiya

AU - Brown, Roger B.

AU - Caffrey, Thomas

AU - Yu, Fang

AU - Johnson, Keith R

AU - Powers, Robert

AU - Hollingsworth, Michael A

AU - Singh, Pankaj

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N2 - Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate growth properties of cancer cells. MUC1 occupies the promoter elements of multiple genes directly involved in glucose metabolism and regulates their expression. Furthermore, MUC1 expression enhances glycolytic activity in pancreatic cancer cells. We also demonstrate that MUC1 expression enhances in vivo glucose uptake and expression of genes involved in glucose uptake and metabolism in orthotopic implantation models of pancreatic cancer. The MUC1 cytoplasmic tail is known to activate multiple signaling pathways through its interactions with several transcription factors/coregulators at the promoter elements of various genes. Our results indicate that MUC1 acts as a modulator of the hypoxic response in pancreatic cancer cells by regulating the expression/stability and activity of hypoxia-inducible factor-1α (HIF-1α). MUC1 physically interacts with HIF-1α and p300 and stabilizes the former at the protein level. By using a ChIP assay, we demonstrate that MUC1 facilitates recruitment of HIF-1α and p300 on glycolytic gene promoters in a hypoxia-dependent manner. Also, by metabolomic studies, we demonstrate that MUC1 regulates multiple metabolite intermediates in the glucose and amino acid metabolic pathways. Thus, our studies indicate that MUC1 acts as a master regulator of the metabolic program and facilitates metabolic alterations in the hypoxic environments that help tumor cells survive and proliferate under such conditions.

AB - Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate growth properties of cancer cells. MUC1 occupies the promoter elements of multiple genes directly involved in glucose metabolism and regulates their expression. Furthermore, MUC1 expression enhances glycolytic activity in pancreatic cancer cells. We also demonstrate that MUC1 expression enhances in vivo glucose uptake and expression of genes involved in glucose uptake and metabolism in orthotopic implantation models of pancreatic cancer. The MUC1 cytoplasmic tail is known to activate multiple signaling pathways through its interactions with several transcription factors/coregulators at the promoter elements of various genes. Our results indicate that MUC1 acts as a modulator of the hypoxic response in pancreatic cancer cells by regulating the expression/stability and activity of hypoxia-inducible factor-1α (HIF-1α). MUC1 physically interacts with HIF-1α and p300 and stabilizes the former at the protein level. By using a ChIP assay, we demonstrate that MUC1 facilitates recruitment of HIF-1α and p300 on glycolytic gene promoters in a hypoxia-dependent manner. Also, by metabolomic studies, we demonstrate that MUC1 regulates multiple metabolite intermediates in the glucose and amino acid metabolic pathways. Thus, our studies indicate that MUC1 acts as a master regulator of the metabolic program and facilitates metabolic alterations in the hypoxic environments that help tumor cells survive and proliferate under such conditions.

KW - 2-Ketoglutarate

KW - Cancer metabolism

KW - Glutamine accumulation

KW - Pentose phosphate pathway

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