MR elastography monitoring of tissue-engineered constructs

Shadi F. Othman, Evan T. Curtis, Sarah A. Plautz, Angela K. Pannier, Stephanie D. Butler, Huihui Xu

Research output: Contribution to journalReview article

29 Citations (Scopus)

Abstract

The objective of tissue engineering (TE) is to create functional replacements for various tissues; the mechanical properties of these engineered constructs are critical to their function. Several techniques have been developed for the measurement of the mechanical properties of tissues and organs; however, current methods are destructive. The field of TE will benefit immensely if biomechanical models developed by these techniques could be combined with existing imaging modalities to enable noninvasive, dynamic assessment of mechanical properties during tissue growth. Specifically, MR elastography (MRE), which is based on the synchronization of a mechanical actuator with a phase contrast imaging pulse sequence, has the capacity to measure tissue strain generated by sonic cyclic displacement. The captured displacement is presented in shear wave images from which the complex shear moduli can be extracted or simplified by a direct measure, termed the shear stiffness. MRE has been extended to the microscopic scale, combining clinical MRE with high-field magnets, stronger magnetic field gradients and smaller, more sensitive, radiofrequency coils, enabling the interrogation of smaller samples, such as tissue-engineered constructs. The following topics are presented in this article: (i) current mechanical measurement techniques and their limitations in TE; (ii) a description of the MRE system, MRE theory and how it can be applied for the measurement of mechanical properties of tissue-engineered constructs; (iii) a summary of in vitro MRE work for the monitoring of osteogenic and adipogenic tissues originating from human adult mesenchymal stem cells (MSCs); (iv) preliminary in vivo studies of MRE of tissues originating from mouse MSCs implanted subcutaneously in immunodeficient mice with an emphasis on in vivo MRE challenges; (v) future directions to resolve current issues with in vivo MRE in the context of how to improve the future role of MRE in TE.

Original languageEnglish (US)
Pages (from-to)452-463
Number of pages12
JournalNMR in Biomedicine
Volume25
Issue number3
DOIs
StatePublished - Mar 1 2012

Fingerprint

Elasticity Imaging Techniques
Tissue
Monitoring
Tissue engineering
Tissue Engineering
Mechanical properties
Stem cells
Mesenchymal Stromal Cells
Mechanical actuators
Imaging techniques
Shear waves
Adult Stem Cells
Magnets
Magnetic Fields
Synchronization
Elastic moduli
Stiffness
Magnetic fields

Keywords

  • Biomechanics
  • Elastography
  • MR elastography
  • Shear wave
  • Tissue engineering

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

Cite this

Othman, S. F., Curtis, E. T., Plautz, S. A., Pannier, A. K., Butler, S. D., & Xu, H. (2012). MR elastography monitoring of tissue-engineered constructs. NMR in Biomedicine, 25(3), 452-463. https://doi.org/10.1002/nbm.1663

MR elastography monitoring of tissue-engineered constructs. / Othman, Shadi F.; Curtis, Evan T.; Plautz, Sarah A.; Pannier, Angela K.; Butler, Stephanie D.; Xu, Huihui.

In: NMR in Biomedicine, Vol. 25, No. 3, 01.03.2012, p. 452-463.

Research output: Contribution to journalReview article

Othman, SF, Curtis, ET, Plautz, SA, Pannier, AK, Butler, SD & Xu, H 2012, 'MR elastography monitoring of tissue-engineered constructs', NMR in Biomedicine, vol. 25, no. 3, pp. 452-463. https://doi.org/10.1002/nbm.1663
Othman SF, Curtis ET, Plautz SA, Pannier AK, Butler SD, Xu H. MR elastography monitoring of tissue-engineered constructs. NMR in Biomedicine. 2012 Mar 1;25(3):452-463. https://doi.org/10.1002/nbm.1663
Othman, Shadi F. ; Curtis, Evan T. ; Plautz, Sarah A. ; Pannier, Angela K. ; Butler, Stephanie D. ; Xu, Huihui. / MR elastography monitoring of tissue-engineered constructs. In: NMR in Biomedicine. 2012 ; Vol. 25, No. 3. pp. 452-463.
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