Movement of DNA across the chloroplast envelope: Implications for the transfer of promiscuous DNA

Heriberto Cerutti, André Jagendorf

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Little is known about the mechanistic basis for the movement of promiscuous nucleic acids across cell membranes. To address this problem we sought conditions that would permit the entry of plasmid DNA into isolated, intact pea chloroplasts. DNA uptake did not occur normally, but was induced by hypotonic treatments, by incubation with millimolar levels of Mg2+, or by heat shock at 42 °C. These results are consistent with DNA movement being permitted by conditions that transiently alter the permeability of the chloroplast envelope. Plant cells are subject to osmotic tensions and/or conditions inducing polymorphic changes in the membranes, such as those used in the present study, under several environmental stresses. In an evolutionary time frame, these phenomena may provide a mechanism for the transfer of promiscuous nucleic acids between organelles.

Original languageEnglish (US)
Pages (from-to)329-337
Number of pages9
JournalPhotosynthesis Research
Volume46
Issue number1-2
DOIs
StatePublished - Nov 1 1995

Fingerprint

Chloroplast DNA
transfer DNA
chloroplast DNA
nucleic acids
chloroplasts
Chloroplasts
Nucleic Acids
DNA
organelles
heat stress
cell membranes
peas
plasmids
permeability
Peas
Plant Cells
Cell membranes
uptake mechanisms
Organelles
Shock

Keywords

  • DNA entry
  • chloroplast envelope
  • promiscuous DNA

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Movement of DNA across the chloroplast envelope : Implications for the transfer of promiscuous DNA. / Cerutti, Heriberto; Jagendorf, André.

In: Photosynthesis Research, Vol. 46, No. 1-2, 01.11.1995, p. 329-337.

Research output: Contribution to journalArticle

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