Mouse interleukin-2 receptor α gene expression. Interleukin-1 and interleukin-2 control transcription via distinct cis-acting elements

P. Sperisen, Ming Wang San Ming Wang, E. Soldaini, M. Pla, C. Rusterholz, P. Bucher, P. Corthesy, P. Reichenbach, M. Nabholz

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have shown that interleukin-1 (IL-1) and IL-2 control IL-2 receptor α (IL-2Rα) gene transcription in CD4-CD8- murine T lymphocyte precursors. Here we map the cis-acting elements that mediate interleukin responsiveness of the mouse IL-2Rα gene using a thymic lymphoma-derived hybridoma (PC60). The transcriptional response of the IL-2Rα gene to stimulation by IL-1 + IL- 2 is biphasic. IL-1 induces a rapid, protein synthesis-independent appearance of IL-2Rα mRNA that is blocked by inhibitors of NF-κB activation. It also primes cells to become IL-2 responsive and thereby prepares the second phase, in which IL-2 induces a 100-fold further increase in IL-2Rα transcripts. Transient transfection experiments show that several elements in the promoter-proximal region of the IL-2Rα gene contribute to IL-1 responsiveness, most importantly an NF-κB site conserved in the human and mouse gene. IL-2 responsiveness, on the other hand, depends on a 78- nucleotide segment 1.3 kilobases upstream of the major transcription start site. This segment functions as an IL-2-inducible enhancer and lies within a region that becomes DNase I hypersensitive in normal T cells in which IL- 2Rα expression has been induced. IL-2 responsiveness requires three distinct elements within the enhancer. Two of these are potential binding sites for STAT proteins.

Original languageEnglish (US)
Pages (from-to)10743-10753
Number of pages11
JournalJournal of Biological Chemistry
Volume270
Issue number18
DOIs
StatePublished - Jan 1 1995

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Interleukin-2 Receptors
Transcription
Interleukin-1
Gene expression
Interleukin-2
Gene Expression
Genes
T-cells
T-Lymphocytes
Interleukins
Transcription Initiation Site
Deoxyribonuclease I
Hybridomas
Genetic Promoter Regions
Transfection
Lymphoma
Proteins
Nucleotides
Chemical activation
Binding Sites

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Mouse interleukin-2 receptor α gene expression. Interleukin-1 and interleukin-2 control transcription via distinct cis-acting elements. / Sperisen, P.; San Ming Wang, Ming Wang; Soldaini, E.; Pla, M.; Rusterholz, C.; Bucher, P.; Corthesy, P.; Reichenbach, P.; Nabholz, M.

In: Journal of Biological Chemistry, Vol. 270, No. 18, 01.01.1995, p. 10743-10753.

Research output: Contribution to journalArticle

Sperisen, P, San Ming Wang, MW, Soldaini, E, Pla, M, Rusterholz, C, Bucher, P, Corthesy, P, Reichenbach, P & Nabholz, M 1995, 'Mouse interleukin-2 receptor α gene expression. Interleukin-1 and interleukin-2 control transcription via distinct cis-acting elements', Journal of Biological Chemistry, vol. 270, no. 18, pp. 10743-10753. https://doi.org/10.1074/jbc.270.18.10743
Sperisen, P. ; San Ming Wang, Ming Wang ; Soldaini, E. ; Pla, M. ; Rusterholz, C. ; Bucher, P. ; Corthesy, P. ; Reichenbach, P. ; Nabholz, M. / Mouse interleukin-2 receptor α gene expression. Interleukin-1 and interleukin-2 control transcription via distinct cis-acting elements. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 18. pp. 10743-10753.
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