Mouse genome editing using the CRISPR/Cas system

Donald W. Harms, Rolen M. Quadros, Davide Seruggia, Masato Ohtsuka, Gou Takahashi, Lluis Montoliu, Channabasavaiah B Gurumurthy

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much more quickly than the previously used techniques, and, more importantly, multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one-cell mouse embryos to create knockout or knock-in mouse models.

Original languageEnglish (US)
Pages (from-to)15.7.1-15.7.27
JournalCurrent Protocols in Human Genetics
Volume83
DOIs
StatePublished - Jan 1 2014

Fingerprint

CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats
Mutation
Medical Genetics
Microinjections
Biomedical Research
Embryonic Structures
Gene Editing

Keywords

  • CRISPR/Cas9
  • Gene editing
  • Mutant mouse
  • Pronuclear and cytoplasmic injection
  • sgRNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Harms, D. W., Quadros, R. M., Seruggia, D., Ohtsuka, M., Takahashi, G., Montoliu, L., & Gurumurthy, C. B. (2014). Mouse genome editing using the CRISPR/Cas system. Current Protocols in Human Genetics, 83, 15.7.1-15.7.27. https://doi.org/10.1002/0471142905.hg1507s83

Mouse genome editing using the CRISPR/Cas system. / Harms, Donald W.; Quadros, Rolen M.; Seruggia, Davide; Ohtsuka, Masato; Takahashi, Gou; Montoliu, Lluis; Gurumurthy, Channabasavaiah B.

In: Current Protocols in Human Genetics, Vol. 83, 01.01.2014, p. 15.7.1-15.7.27.

Research output: Contribution to journalArticle

Harms, DW, Quadros, RM, Seruggia, D, Ohtsuka, M, Takahashi, G, Montoliu, L & Gurumurthy, CB 2014, 'Mouse genome editing using the CRISPR/Cas system', Current Protocols in Human Genetics, vol. 83, pp. 15.7.1-15.7.27. https://doi.org/10.1002/0471142905.hg1507s83
Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L et al. Mouse genome editing using the CRISPR/Cas system. Current Protocols in Human Genetics. 2014 Jan 1;83:15.7.1-15.7.27. https://doi.org/10.1002/0471142905.hg1507s83
Harms, Donald W. ; Quadros, Rolen M. ; Seruggia, Davide ; Ohtsuka, Masato ; Takahashi, Gou ; Montoliu, Lluis ; Gurumurthy, Channabasavaiah B. / Mouse genome editing using the CRISPR/Cas system. In: Current Protocols in Human Genetics. 2014 ; Vol. 83. pp. 15.7.1-15.7.27.
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