Morphology and Proteome Characterization of the Salivary Glands of the Western Chinch Bug (Hemiptera: Blissidae)

Crystal Ramm, Astri Wayadande, Lisa Baird, Renu Nandakumar, Nandakumar Madayiputhiya, Keenan Amundsen, Teresa Donze-Reiner, Frederick Baxendale, Gautam Sarath, Tiffany Heng-Moss

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The western chinch bug, Blissus occiduus Barber, is a serious pest of buffalograss, Buchloe dactyloides (Nuttall) due to physical and chemical damage caused during the feeding process. Although previous work has investigated the feeding behaviors of chinch bugs in the Blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. Whole and sectioned B. occiduus salivary glands were visualized using light and scanning electron microscopy to determine overall structure and cell types of the salivary glands and their individual lobes. Microscopy revealed a pair of trilobed principal glands and a pair of tubular accessory glands of differing cellular types. To link structure with function, the salivary gland proteome was characterized using liquid chromatography tandem mass spectrometry. The salivary proteome analysis resulted in B. occiduus sequences matching 228 nonhomologous protein sequences of the pea aphid, Acyrthosiphon pisum (Harris), with many specific to the proteins present in the salivary proteome of A. pisum. A number of sequences were assigned the molecular function of hydrolase and oxido-reductase activity, with one specific protein sequence revealing a peroxidase-like function. This is the first study to characterize the salivary proteome of B. occiduus and the first of any species in the family Blissidae.

Original languageEnglish (US)
Pages (from-to)2055-2064
Number of pages10
JournalJournal of economic entomology
Volume108
Issue number4
DOIs
StatePublished - Jan 1 2015

Fingerprint

Blissus occiduus
Blissidae
proteome
salivary glands
Hemiptera
Acyrthosiphon pisum
protein
Bouteloua dactyloides
Blissus
feeding behavior
aphid
amino acid sequences
Blissus leucopterus
liquid chromatography
microscopy
mass spectrometry
scanning electron microscopy
insect
hydrolases
damage

Keywords

  • Hemiptera
  • chinch bug
  • salivary gland
  • salivary proteome

ASJC Scopus subject areas

  • Ecology
  • Insect Science

Cite this

Ramm, C., Wayadande, A., Baird, L., Nandakumar, R., Madayiputhiya, N., Amundsen, K., ... Heng-Moss, T. (2015). Morphology and Proteome Characterization of the Salivary Glands of the Western Chinch Bug (Hemiptera: Blissidae). Journal of economic entomology, 108(4), 2055-2064. https://doi.org/10.1093/jee/tov149

Morphology and Proteome Characterization of the Salivary Glands of the Western Chinch Bug (Hemiptera : Blissidae). / Ramm, Crystal; Wayadande, Astri; Baird, Lisa; Nandakumar, Renu; Madayiputhiya, Nandakumar; Amundsen, Keenan; Donze-Reiner, Teresa; Baxendale, Frederick; Sarath, Gautam; Heng-Moss, Tiffany.

In: Journal of economic entomology, Vol. 108, No. 4, 01.01.2015, p. 2055-2064.

Research output: Contribution to journalArticle

Ramm, C, Wayadande, A, Baird, L, Nandakumar, R, Madayiputhiya, N, Amundsen, K, Donze-Reiner, T, Baxendale, F, Sarath, G & Heng-Moss, T 2015, 'Morphology and Proteome Characterization of the Salivary Glands of the Western Chinch Bug (Hemiptera: Blissidae)', Journal of economic entomology, vol. 108, no. 4, pp. 2055-2064. https://doi.org/10.1093/jee/tov149
Ramm, Crystal ; Wayadande, Astri ; Baird, Lisa ; Nandakumar, Renu ; Madayiputhiya, Nandakumar ; Amundsen, Keenan ; Donze-Reiner, Teresa ; Baxendale, Frederick ; Sarath, Gautam ; Heng-Moss, Tiffany. / Morphology and Proteome Characterization of the Salivary Glands of the Western Chinch Bug (Hemiptera : Blissidae). In: Journal of economic entomology. 2015 ; Vol. 108, No. 4. pp. 2055-2064.
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