Morphological and physical analysis of natural phospholipids-based biomembranes

Adrien Jacquot, Grégory Francius, Angelina Razafitianamaharavo, Fariba Dehghani, Ali Tamayol, Michel Linder, Elmira Arab-Tehrany

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Liposomes are currently an important part of biological, pharmaceutical, medical and nutritional research, as they are considered to be among the most effective carriers for the introduction of various types of bioactive agents into target cells. Scope of Review: In this work, we study the lipid organization and mechanical properties of biomembranes made of marine and plant phospholipids. Membranes based on phospholipids extracted from rapeseed and salmon are studied in the form of liposome and as supported lipid bilayer. Dioleylphosphatidylcholine (DOPC) and dipalmitoylphosphatidylcholine (DPPC) are used as references to determine the lipid organization of marine and plant phospholipid based membranes. Atomic force microscopy (AFM) imaging and force spectroscopy measurements are performed to investigate the membranes' topography at the micrometer scale and to determine their mechanical properties. Major Conclusions: The mechanical properties of the membranes are correlated to the fatty acid composition, the morphology, the electrophoretic mobility and the membrane fluidity. Thus, soft and homogeneous mechanical properties are evidenced for salmon phospholipids membrane containing various polyunsaturated fatty acids. Besides, phase segregation in rapeseed membrane and more important mechanical properties were emphasized for this type of membranes by contrast to the marine phospholipids based membranes. General Significance: This paper provides new information on the nanomechanical and morphological properties of membrane in form of liposome by AFM. The originality of this work is to characterize the physico-chemical properties of the nanoliposome from the natural sources containing various fatty acids and polar head.

Original languageEnglish (US)
Article numbere107435
JournalPloS one
Volume9
Issue number9
DOIs
StatePublished - Sep 19 2014

Fingerprint

mechanical properties
Phospholipids
phospholipids
Membranes
atomic force microscopy
rapeseed
salmon
Liposomes
Mechanical properties
Brassica rapa
Atomic Force Microscopy
Salmon
lipid bilayers
membrane fluidity
lipids
topography
Fatty Acids
polyunsaturated fatty acids
spectroscopy
Atomic force microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Jacquot, A., Francius, G., Razafitianamaharavo, A., Dehghani, F., Tamayol, A., Linder, M., & Arab-Tehrany, E. (2014). Morphological and physical analysis of natural phospholipids-based biomembranes. PloS one, 9(9), [e107435]. https://doi.org/10.1371/journal.pone.0107435

Morphological and physical analysis of natural phospholipids-based biomembranes. / Jacquot, Adrien; Francius, Grégory; Razafitianamaharavo, Angelina; Dehghani, Fariba; Tamayol, Ali; Linder, Michel; Arab-Tehrany, Elmira.

In: PloS one, Vol. 9, No. 9, e107435, 19.09.2014.

Research output: Contribution to journalArticle

Jacquot, A, Francius, G, Razafitianamaharavo, A, Dehghani, F, Tamayol, A, Linder, M & Arab-Tehrany, E 2014, 'Morphological and physical analysis of natural phospholipids-based biomembranes', PloS one, vol. 9, no. 9, e107435. https://doi.org/10.1371/journal.pone.0107435
Jacquot A, Francius G, Razafitianamaharavo A, Dehghani F, Tamayol A, Linder M et al. Morphological and physical analysis of natural phospholipids-based biomembranes. PloS one. 2014 Sep 19;9(9). e107435. https://doi.org/10.1371/journal.pone.0107435
Jacquot, Adrien ; Francius, Grégory ; Razafitianamaharavo, Angelina ; Dehghani, Fariba ; Tamayol, Ali ; Linder, Michel ; Arab-Tehrany, Elmira. / Morphological and physical analysis of natural phospholipids-based biomembranes. In: PloS one. 2014 ; Vol. 9, No. 9.
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