Morphogenesis and regulation of Bergmann glial processes during Purkinje cell dendritic spine ensheathment and synaptogenesis

Jocelyn J. Lippman, Tamar Lordkipanidze, Margaret E. Buell, Sung Ok Yoon, Anna Dunaevsky

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Astrocytes have an important role in synaptic formation and function but how astrocytic processes become associated with synaptic structures during development is not well understood. Here we analyzed the pattern of growth of the processes extending off the main Bergmann glial (BG) shafts during synaptogenesis in the cerebellum. We found that during this period, BG process outgrowth was correlated with increased ensheathment of dendritic spines. In addition, two-photon time-lapse imaging revealed that BG processes were highly dynamic, and processes became more stable as the period of spine ensheathment progressed. While process motility was dependent on actin polymerization, activity of cytoskeletal regulators Rac1 and RhoG did not play a role in glial process dynamics or density, but was critical for maintaining process length. We extended this finding to probe the relationship between process morphology and ensheathment, finding that shortened processes result in decreased coverage of the spine. Furthermore, we found that areas in which BG expressed dn-Rac1, and therefore had a lower level of synaptic ensheathment, showed an overall increase in synapse number. These analyses reveal how BG processes grow to surround synaptic structures, elucidate the importance of BG process structure for proper development of synaptic ensheathment, and reveal a role for ensheathment in synapse formation.

Original languageEnglish (US)
Pages (from-to)1463-1477
Number of pages15
JournalGlia
Volume56
Issue number13
DOIs
StatePublished - Oct 1 2008

Fingerprint

Dendritic Spines
Purkinje Cells
Morphogenesis
Neuroglia
Synapses
Spine
Time-Lapse Imaging
Photons
Astrocytes
Polymerization
Cerebellum
Actins
Growth

Keywords

  • Bergmann glia
  • Cerebellum
  • Dendritic spines
  • Purkinje cell
  • Rac1
  • Synaptogenesis

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Morphogenesis and regulation of Bergmann glial processes during Purkinje cell dendritic spine ensheathment and synaptogenesis. / Lippman, Jocelyn J.; Lordkipanidze, Tamar; Buell, Margaret E.; Yoon, Sung Ok; Dunaevsky, Anna.

In: Glia, Vol. 56, No. 13, 01.10.2008, p. 1463-1477.

Research output: Contribution to journalArticle

Lippman, Jocelyn J. ; Lordkipanidze, Tamar ; Buell, Margaret E. ; Yoon, Sung Ok ; Dunaevsky, Anna. / Morphogenesis and regulation of Bergmann glial processes during Purkinje cell dendritic spine ensheathment and synaptogenesis. In: Glia. 2008 ; Vol. 56, No. 13. pp. 1463-1477.
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