Morphine Potentiates Dysbiotic Microbial and Metabolic Shifts in Acute SIV Infection

Gregory M. Sindberg, Shannon E. Callen, Santanu Banerjee, Jingjing Meng, Vanessa L. Hale, Ramakrishna Hegde, Paul D. Cheney, Francois Villinger, Sabita Roy, Shilpa J Buch

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human Immunodeficiency Virus (HIV) pathogenesis has been closely linked with microbial translocation, which is believed to drive inflammation and HIV replication. Opioid drugs have been shown to worsen this symptom, leading to a faster progression of HIV infection to Acquired Immunodeficiency Syndrome (AIDS). The interaction of HIV and opioid drugs has not been studied at early stages of HIV, particularly in the gut microbiome where changes may precede translocation events. This study modeled early HIV infection by examining Simian Immunodeficiency Virus (SIV)-infected primates at 21 days or less both independently and in the context of opioid use. Fecal samples were analyzed both for 16S analysis of microbial populations as well as metabolite profiles via mass spectrometry. Our results indicate that changes are minor in SIV treated animals in the time points examined, however animals treated with morphine and SIV had significant changes in their microbial communities and metabolic profiles. This occurred in a time-independent fashion with morphine regardless of how long the animal had morphine in its system. Globally, the observed changes support that microbial dysbiosis is occurring in these animals at an early time, which likely contributes to the translocation events observed later in SIV/HIV pathogenesis. Additionally, metabolic changes were predictive of specific treatment groups, which could be further developed as a diagnostic tool or future intervention target to overcome and slow the progression of HIV infection to AIDS.

Original languageEnglish (US)
Pages (from-to)200-214
Number of pages15
JournalJournal of Neuroimmune Pharmacology
Volume14
Issue number2
DOIs
StatePublished - Jun 15 2019

Fingerprint

Simian Immunodeficiency Virus
Virus Diseases
Morphine
HIV
Opioid Analgesics
Acquired Immunodeficiency Syndrome
Dysbiosis
Metabolome
Virus Replication
Pharmaceutical Preparations
Primates
Mass Spectrometry
Inflammation

Keywords

  • HIV pathogenesis
  • Intestine metabolism
  • Intestine microbiome
  • Non-human primates
  • Opioids
  • SIV

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Morphine Potentiates Dysbiotic Microbial and Metabolic Shifts in Acute SIV Infection. / Sindberg, Gregory M.; Callen, Shannon E.; Banerjee, Santanu; Meng, Jingjing; Hale, Vanessa L.; Hegde, Ramakrishna; Cheney, Paul D.; Villinger, Francois; Roy, Sabita; Buch, Shilpa J.

In: Journal of Neuroimmune Pharmacology, Vol. 14, No. 2, 15.06.2019, p. 200-214.

Research output: Contribution to journalArticle

Sindberg, GM, Callen, SE, Banerjee, S, Meng, J, Hale, VL, Hegde, R, Cheney, PD, Villinger, F, Roy, S & Buch, SJ 2019, 'Morphine Potentiates Dysbiotic Microbial and Metabolic Shifts in Acute SIV Infection', Journal of Neuroimmune Pharmacology, vol. 14, no. 2, pp. 200-214. https://doi.org/10.1007/s11481-018-9805-6
Sindberg, Gregory M. ; Callen, Shannon E. ; Banerjee, Santanu ; Meng, Jingjing ; Hale, Vanessa L. ; Hegde, Ramakrishna ; Cheney, Paul D. ; Villinger, Francois ; Roy, Sabita ; Buch, Shilpa J. / Morphine Potentiates Dysbiotic Microbial and Metabolic Shifts in Acute SIV Infection. In: Journal of Neuroimmune Pharmacology. 2019 ; Vol. 14, No. 2. pp. 200-214.
@article{371a71eebebc4188a7067946d9a7a634,
title = "Morphine Potentiates Dysbiotic Microbial and Metabolic Shifts in Acute SIV Infection",
abstract = "Human Immunodeficiency Virus (HIV) pathogenesis has been closely linked with microbial translocation, which is believed to drive inflammation and HIV replication. Opioid drugs have been shown to worsen this symptom, leading to a faster progression of HIV infection to Acquired Immunodeficiency Syndrome (AIDS). The interaction of HIV and opioid drugs has not been studied at early stages of HIV, particularly in the gut microbiome where changes may precede translocation events. This study modeled early HIV infection by examining Simian Immunodeficiency Virus (SIV)-infected primates at 21 days or less both independently and in the context of opioid use. Fecal samples were analyzed both for 16S analysis of microbial populations as well as metabolite profiles via mass spectrometry. Our results indicate that changes are minor in SIV treated animals in the time points examined, however animals treated with morphine and SIV had significant changes in their microbial communities and metabolic profiles. This occurred in a time-independent fashion with morphine regardless of how long the animal had morphine in its system. Globally, the observed changes support that microbial dysbiosis is occurring in these animals at an early time, which likely contributes to the translocation events observed later in SIV/HIV pathogenesis. Additionally, metabolic changes were predictive of specific treatment groups, which could be further developed as a diagnostic tool or future intervention target to overcome and slow the progression of HIV infection to AIDS.",
keywords = "HIV pathogenesis, Intestine metabolism, Intestine microbiome, Non-human primates, Opioids, SIV",
author = "Sindberg, {Gregory M.} and Callen, {Shannon E.} and Santanu Banerjee and Jingjing Meng and Hale, {Vanessa L.} and Ramakrishna Hegde and Cheney, {Paul D.} and Francois Villinger and Sabita Roy and Buch, {Shilpa J}",
year = "2019",
month = "6",
day = "15",
doi = "10.1007/s11481-018-9805-6",
language = "English (US)",
volume = "14",
pages = "200--214",
journal = "Journal of NeuroImmune Pharmacology",
issn = "1557-1890",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Morphine Potentiates Dysbiotic Microbial and Metabolic Shifts in Acute SIV Infection

AU - Sindberg, Gregory M.

AU - Callen, Shannon E.

AU - Banerjee, Santanu

AU - Meng, Jingjing

AU - Hale, Vanessa L.

AU - Hegde, Ramakrishna

AU - Cheney, Paul D.

AU - Villinger, Francois

AU - Roy, Sabita

AU - Buch, Shilpa J

PY - 2019/6/15

Y1 - 2019/6/15

N2 - Human Immunodeficiency Virus (HIV) pathogenesis has been closely linked with microbial translocation, which is believed to drive inflammation and HIV replication. Opioid drugs have been shown to worsen this symptom, leading to a faster progression of HIV infection to Acquired Immunodeficiency Syndrome (AIDS). The interaction of HIV and opioid drugs has not been studied at early stages of HIV, particularly in the gut microbiome where changes may precede translocation events. This study modeled early HIV infection by examining Simian Immunodeficiency Virus (SIV)-infected primates at 21 days or less both independently and in the context of opioid use. Fecal samples were analyzed both for 16S analysis of microbial populations as well as metabolite profiles via mass spectrometry. Our results indicate that changes are minor in SIV treated animals in the time points examined, however animals treated with morphine and SIV had significant changes in their microbial communities and metabolic profiles. This occurred in a time-independent fashion with morphine regardless of how long the animal had morphine in its system. Globally, the observed changes support that microbial dysbiosis is occurring in these animals at an early time, which likely contributes to the translocation events observed later in SIV/HIV pathogenesis. Additionally, metabolic changes were predictive of specific treatment groups, which could be further developed as a diagnostic tool or future intervention target to overcome and slow the progression of HIV infection to AIDS.

AB - Human Immunodeficiency Virus (HIV) pathogenesis has been closely linked with microbial translocation, which is believed to drive inflammation and HIV replication. Opioid drugs have been shown to worsen this symptom, leading to a faster progression of HIV infection to Acquired Immunodeficiency Syndrome (AIDS). The interaction of HIV and opioid drugs has not been studied at early stages of HIV, particularly in the gut microbiome where changes may precede translocation events. This study modeled early HIV infection by examining Simian Immunodeficiency Virus (SIV)-infected primates at 21 days or less both independently and in the context of opioid use. Fecal samples were analyzed both for 16S analysis of microbial populations as well as metabolite profiles via mass spectrometry. Our results indicate that changes are minor in SIV treated animals in the time points examined, however animals treated with morphine and SIV had significant changes in their microbial communities and metabolic profiles. This occurred in a time-independent fashion with morphine regardless of how long the animal had morphine in its system. Globally, the observed changes support that microbial dysbiosis is occurring in these animals at an early time, which likely contributes to the translocation events observed later in SIV/HIV pathogenesis. Additionally, metabolic changes were predictive of specific treatment groups, which could be further developed as a diagnostic tool or future intervention target to overcome and slow the progression of HIV infection to AIDS.

KW - HIV pathogenesis

KW - Intestine metabolism

KW - Intestine microbiome

KW - Non-human primates

KW - Opioids

KW - SIV

UR - http://www.scopus.com/inward/record.url?scp=85053814204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053814204&partnerID=8YFLogxK

U2 - 10.1007/s11481-018-9805-6

DO - 10.1007/s11481-018-9805-6

M3 - Article

C2 - 30242614

AN - SCOPUS:85053814204

VL - 14

SP - 200

EP - 214

JO - Journal of NeuroImmune Pharmacology

JF - Journal of NeuroImmune Pharmacology

SN - 1557-1890

IS - 2

ER -